Entering edit mode
4 months ago
analyst
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50
Hi all,
I am analysing NGS amplicon reads of crisper gene samples. I do not know exactly which genes do they belong to because if I align reads against gene sequence (that was supposed to be) using BWA it gives 0% identity however by aligning reference genome I get 99% alignment rate. Now how can I find about the gene through reads data. Is there any way to find through sam or bam file that which chromosomal position are maximum reads aligning to?
Thanks
Thanks Pierre!
I got this output
Can you please interpret the output
Okay sure Pierre I will take care of it next time
the max depth is 3520 at chr1:80660513
Thanks for the interpretation Pierre!