News:Online course: An Introduction to Nanopore Direct RNA Sequencing
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4 weeks ago
carlopecoraro2 ★ 2.5k

Dear all,

We're thrilled to announce our upcoming course: An Introduction to Nanopore Direct RNA Sequencing.

Dates: 7-10 October 2024 Location: Online, fostering international participation

Overview: Nanopore direct RNA Sequencing represents a groundbreaking leap in the field, enabling the direct sequencing of full-length, native RNA molecules without the need for retrotranscription or amplification. This revolutionary technology opens doors to a plethora of advantages, including simplified RNA isoform detection, precise polyA tail length measurement, and identification of RNA modifications. However, harnessing the power of Nanopore data demands specialized skills and tools.

Our comprehensive 4-day course is designed to equip researchers with the theoretical knowledge and practical expertise necessary to navigate the intricacies of direct RNA-Seq analysis. From fundamental concepts to advanced techniques, participants will explore everything from data QC and gene expression quantification to transcriptome assembly and RNA modification detection.

Target Audience and Assumed Background: This course is tailored for researchers with a foundational understanding of RNA sequencing concepts. While prior bioinformatics training is not mandatory, familiarity with command-line tools and *nix environments (e.g., Linux or MacOS) is highly beneficial.

Program Highlights:

  • Monday: Theoretical Introduction to Nanopore Direct RNA-Sequencing
  • Tuesday: First Steps in dRNA Analysis: Demultiplexing, Basecalling, and Read Mapping
  • Wednesday: Exploiting Single Molecule Resolution: Tail Length Measurement and RNA Modification Detection
  • Thursday: Individual Assignments and Conclusions

Why Attend?

  • Gain hands-on experience with state-of-the-art analysis tools.
  • Interact with leading experts in the field.
  • Analyze real-world datasets and receive personalized guidance.


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