qPCR gene expression data analysis
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27 days ago
ebertomeup • 0

Hi people,

I am new here. I have analyzed the expression of two genes of interest with qPCR. The samples come from two groups (A and B) and each group is subjected to two conditions (Infected and Control). What I want to see is if there are significant differences in the expression of these two genes, between the experimental groups. I do NOT want to see if there is interaction between them. So I have thought of making a nested model for each of the genes (make separate subsets), since the levels of the condition variable are found in both groups: aov(gene expression value of gene 1 ~ group + condition%in%group.

How do you see it, would you do it differently?

Edgar

qPCR geneexpression anova • 159 views
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