Entering edit mode
9 weeks ago
dawnyipingzou
•
0
After processing the scTCR-seq or scBCR-seq data using cellranger, I discovered that the concatenated sequence of any contig (fwr1+cdr1+fwr2+cdr2+fwr3+cdr3+fwr4), extracted from the all_contig_annotations.csv, is shorter than the sequence in all_contig.fasta. Specifically, it appears that all_contig_annotations.csv excludes the first fragment of the fasta sequence.
My question is, if I only have access to the all_contig_annotations.csv and don't have the raw data, but I require the complete sequence to perform igblastn, is there a method to recover the entire sequence from all_contig_annotations.csv? Thanks in advance.
