Rescaling normalized enrichment score (NES)
Entering edit mode
4 weeks ago
CTLong ▴ 120

Hi all,

I performed VIPER analysis to obtain transcription factor activity normalized enrichment scores (NES) corresponding my samples. I would like to plot a heatmap to show the difference in NES for multiple transcription factors. However, I have come across this problem whereby the range of NES goes from -15 to 15, but the NES difference between my two groups of samples are around 1 to 2. As a result, when I plot a heatmap, the activity differences between the groups are not obvious.

Is there a way to rescale the NES where it can be justified? I have thought about calculating the NES zscore or log normalization of the absolute NES score. Are these justifiable? Thanks.

heatmap • 126 views

Login before adding your answer.

Traffic: 1455 users visited in the last hour
Help About
Access RSS

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6