NO sequence type of bacteria revealed using mlst package
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14 months ago

I have applied mlst command to get the sequence type of a bunch of 155 assembly of klebsiella bacteria . It was successful in all of them except for 38 entry which did not reveal any ST although there was number assigned to the 7-genes. when I inspected the messages appeared in those negative ones, there was a warning saying: duplicate allele were also recovered in Klebsiella and E. coli. So, I took these assembly, and uploaded them on the center for genomic epidemiology (https://www.genomicepidemiology.org/services/), shere it gave me their ST. Could you please advice on how to tackle this in linux ? are there any other tool of mlst to retrieve the ST ?

this is was my code for some that did not reveal any ST:

mlst GCA_036986515.fasta
mlst GCF_004127345.fasta
mlst GCF_004127375.fasta

For some assemblies that gave a ST

mlst GCF_004127595.fasta
mlst GCF_004127885.fasta
Assembly Sequencing Genomics • 395 views
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