Entering edit mode
13 months ago
aaron.varghese
•
0
I need to use dba.contrast to compare 4 tissue groups. I'll use the example from the vignette:
ID Tissue Factor Condition Treatment Replicate Reads FRiP
1 BT4741 BT474 ER Resistant Full-Media 1 652697 0.16
2 BT4742 BT474 ER Resistant Full-Media 2 663370 0.15
3 MCF71 MCF7 ER Responsive Full-Media 1 346429 0.31
4 MCF72 MCF7 ER Responsive Full-Media 2 368052 0.19
5 MCF73 MCF7 ER Responsive Full-Media 3 466273 0.25
6 T47D1 T47D ER Responsive Full-Media 1 399879 0.11
7 T47D2 T47D ER Responsive Full-Media 2 1475415 0.06
8 MCF7r1 MCF7 ER Resistant Full-Media 1 616630 0.22
9 MCF7r2 MCF7 ER Resistant Full-Media 2 593224 0.14
10 ZR751 ZR75 ER Responsive Full-Media 1 706836 0.33
11 ZR752 ZR75 ER Responsive Full-Media 2 2575408 0.22
I want to contrast all five groups in the "Tissue" column, analyze, then plot on a heatmap so all five groups are contrasted. How can I accomplish this?
