Post-sequencing basecalling error in MinKNOW

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7 months ago

Cedrick • 0

Hello.

I have been trying to do post-sequencing analysis (i.e., basecalling) in MinKNOW using the raw reads I generated from my sequencing run. However, it always gives me this error:

2025-01-28 14:23:04.442677 [ont/error] boost::filesystem::directory_iterator::construct: Operation not permitted [system:1]: "/Users/cedrickalguzar/Downloads/queued_reads/complete_reads_f025be2b-1b99-428a-82eb-02f6839905ce"
2025-01-28 14:23:04.442701 [ont/warning] An error has occurred. Aborting.

Does anyone here know how to troubleshoot this? Thank you!

minknow dorado long reads nanopore • 588 views

ADD COMMENT • link 7 months ago by Cedrick • 0

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Since you added the dorado tag are you directly using dorado to recall bases or are you using the MinKNOW GUI? You will need POD5/fast5 files to recall bases using a different accuracy model. Do you have those available?

If that is not applicable you may want to contact ONT support and see if they can help.

ADD REPLY • link 7 months ago by GenoMax 153k

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I am using MinKNOW GUI for my post-sequencing basecalling and the data that I have been trying to load are all POD5 files.

ADD REPLY • link 7 months ago by Cedrick • 0

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