Entering edit mode
7 months ago
Major ginger
•
0
Hi all,
I have been using R.9.4.1 flongle flow cell for 16srRNA sequencing. However, upon updating the minKNOW software we are not getting results. A significant portion of the reads (183.92k out of 222.51k) are classified as failed reads. Many pores became inactive or unavailable during the run (from 46 pores at the start to 11 pores at the end), Most barcodes had no passed reads.
I realize that I should switch to newer version R10, but any possible suggestions to make things works? Till 18th November 24, we were getting consistent barcodes reads. Also, my samples are now barcoded using old kit and now I have no other option than to make it R.9.4.1 work.
HELP!
Please contact ONT tech support and/or your local nanopore field applications scientist. They would likely be able to take a look at your data/setup and diagnose.