How to convert .ab1 to fastq

Question

[dr.suvarnakapale]

Good afternoon even

I actually need a help regarding How to convert ".ab1" file taken from sanger sequencing platform to its "fastq" file, with the original quality scoring for each based calls

I am doing it using chromas, SnapGene, Galaxy tools but I am not getting the correct form of fastq file could anybody please suggest me?

Or I have a doubt like whether it can be converted or not once after getting .ab1 file to fastq?

Answer 1

[Pierre Lindenbaum]

AB1 to FASTQ

Answer 2

[size_t]

try this gui tool: https://technelysium.com.au/wp/chromas/