Tool for Estimation of Purity and Ploidy in Precancerous Lesions

Question

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Hi,

I have WES data from precancer lesions and paired tumors needed to estimate tumor purity and ploidy. Crucially, I do not have match normal samples. However, I have a Panel of Normals (PoN) from unmatched blood samples.

I've seen tools like Sequenza used in similar contexts (e.g. Genomic comparison of esophageal squamous cell carcinoma and its precursor lesions by multi-region whole-exome sequencing), but they require matched normals.

Questions:

Is ABSOLUTE suitable for estimating purity/ploidy from precancerous lesion WES data in this scenario?

If not, or if there are better alternatives, what tools would you recommend for my data?

Thanks for any guidance!