Insert size is zero

Question

[SpliceAndScript]

In my fastp reports I observed that in some of the samples the insert peak size was zero. What could be the possible reason for it? Is it due to small insert size and r1 r2 are overlapping during fastp analysis?? or is it due to some inefficiency in fastp v0.23.4 to accurately detect insert size??

Answer 1

[GenoMax]

It is possible, if your have libraries that failed and are just adapter dimers.

You could also try bbmerge.sh from BBMap suite to get an estimate: BBMap and BBmerge insert sizs