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6.3 years ago
El Niño
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10
Hi everyone,
I am a bioinformatics newbie. I was asked to draw a genome map of a strain of bacteria whose complete genome sequence has been worked out. However, when I put my .fasta file onto the website CGView server, it showed only the longest contig (~280KB) on the circular genome map, while in fact my whole genome is around 5.8MB long.
What happened? Does anybody know how to solve this problem? Thank you a bunch. The genome assembly part was done by another lab using SOAP denovo. The assembly results give 201 contigs.
use another tool like circos ?
Yeah, should be that. But I still need to know why my data cannot generate a full genome map? Do you have any idea?
I am also drawing bacterial cicular genome map based on WGS of more than 1000 contigs. Which tools should I use for drawing bacterial cicular map capable able of being used for paper publication. Thanks for your kind supports!
Circular genome maps of unassembled contigs do not make much sense, unfortunately. Anyway,
The only honest and decent way is to finish the assembly: assemble the contigs into complete genome sequence(s). If there are no plasmids in the genome, then it should be a single (typically circular) chromosome sequence.