ONT bulk PCR cDNA protocol

Question

[Sowmya]

Is UMI ligation typically used in bulk ONT PCR cDNA sequencing? If not, how is deduplication handled given the risk of false positives from full-length transcript duplicates?

Answer 1

[GenoMax]

https://nanoporetech.com/document/pcr-cdna-sequencing-v14-sqk-pcs114 cDNA protocol incorporates UMI's.

It is for https://store.nanoporetech.com/us/cdna-pcr-sequencing-kit-v14.html cDNA-PCR kit.