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20 days ago
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Hello, I am currently registering my WGS phage with NCBI/Banklt and I am facing a significant issue. I have submitted the FASTA file containing all the genes, following the site's instructions, along with the feature table. Unfortunately, I am encountering an error for each gene. For instance, the sequence length for gene number 1 is reported as 507. However, when I subtract the starting point (290) from the ending point (796), I calculate the length as
- The BLAST results for each gene also indicate a length of 507, which aligns with the site's information. This discrepancy between the calculated length (506) and the expected length (507) is leading to the error. I have carefully reviewed the gene and found no issues. Could you please advise me on resolving this error without altering the reading frame? Thank you for your assistance.
