Entering edit mode
10 hours ago
danielpintard
▴
10
Hey everyone! I have been working with some VisiumHD data and ran Bin2Cell on it to group the 2 µm bins into larger bins that reflect single cells. After I ran the B2C pipeline, I took my new, deconvolved spatial data object and ran a standard scRNA-seq analysis workflow. When I calculated QC metrics, I noticed this strange, sort of banding pattern that occurs in the plot (seen below). I assume this may be an artefact of the B2C pipeline, but I am pretty lost as to what could cause this. I just have never seen a pct_count_mt
plot like this before! Would love to hear others ideas and hypotheses!
Update: take a look at the plot below