Hi,
I am currently re-processing Digital Spatial Profiling (Whole Transcriptome Atlas)
data starting from Fastq. In order to run GeoMx NGS pipeline
to convert this into DCC format, I need a configuration file in the ini
format. I was wondering whether each specific experiment comes with its unique ini configuration, or whether there is a general configuration for whole transcriptome atlas
which I can download. I noticed that the pkc
configuration can be downloaded from the Nanstrong official website here. But I'm not sure whether this pkc configuration is what the pipeline requires as input. Thanks for the help!
Generally did this with the entire flowcell folder and not fastq files. The data needs to be demultiplexed in a way that the fastq files are named with
DSP*
sample names that are in the samplesheet for the experiment.Never looked inside these files but an experiment specific samplesheet (for demultiplexing) and the
.ini
file were provided in a.zip
readout package for each GeoMX run. The samplesheet was needed to run the demultiplexing since it had theDSP*
names for the samples that end up on the fastq files.