I'm looking for some advice from anyone who has experience of using simulated playback or specifically Readfish for selective sequencing (a.k.a. adaptive sampling) on ONT. I am currently trying to test the simulated playback feature (using a simulated Minion device as I don't have permanent access to an actual device currently).
The issue I am having currently is with getting the simulated playback to work. Does anyone know of documentation/have a protocol for how to do this? I am following the steps on the Readfish readme, and cannot find any ONT documentation on this feature of Minknow, but currently receiving a very unhelpful error (MinION Mk1B ยท MS00000 20 October, 10:18:43 Unable to open Playback file '' as a source for playback. -- this is despite selecting a bulk FAST5 file downloaded from the Readfish GitHub page provided expressly for this purpose).
Thanks in advance!
Which version of MinKNOW are you using? Have you seen the requirement of
doradoandMinKNOWcombinations noted onreadfishsite.Adaptive sampling can be a tricky thing. Some people report having good success where as for others it is an exercise in frustration. You may not know until you actually try it out with your own libraries.