Entering edit mode
                    4 hours ago
        matt_arnold_bio
        
    
        •
    
    0
    I am looking to try out some different methods for ONT adaptive sampling, starting by running some playback experiments. Anyone know where I can download some bulk fast5 files? Specifically interested in viral sequences.
Here is one SRA project that uses "adaptive sampling": https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1314197 Looking at the data for a couple of samples, they do not seem to provide
fast5files.Here are some random examples of
fast5datasets (they are not for adaptive sampling) :ONT makes some test datasets available (at least one contains fast5 files): https://registry.opendata.aws/ont-open-data/
Some test data here: https://figshare.com/articles/dataset/Example_dataset_containing_Fast5_and_BAM_files/26093869?file=48273898
You will also find
fast5files inData Accesstab of some SRA datasets (depending on if the submitters have posted them). Here is one example: https://trace.ncbi.nlm.nih.gov/Traces/index.html?view=run_browser&acc=SRR8767349&display=data-accessfast5is a legacy file format. POD5 is the current format in use: https://nanoporetech.com/document/data-analysis#file-formatsWere you not able to make the test data on
readfishsite work?