Moderator: lh3
lh3 ♦ 30k
- Reputation:
- 30,380
- Status:
- Trusted
- Location:
- United States
- Last seen:
- 3 months, 2 weeks ago
- Joined:
- 7 years, 5 months ago
- Email:
- l**@temp.me.com
Posts by lh3
<prev
• 1,495 results •
page 1 of 150 •
next >
0
votes
9
answers
2.9k
views
9
answers
... I can understand why you say yours is a paradigm shift: optional columns unify multiple formats, but optional tags don't. If you had implemented this 20 years ago and a toolbox working with it as efficiently as other unix tools, your proposal might have greatly influenced bio format design – I can't ...
written 9 months ago by
lh3 ♦ 30k
2
votes
9
answers
2.9k
views
9
answers
... My understanding is that to an alignment format, you still propose to have fixed columns and optional columns. The major difference from SAM would be that each line ought to have a fixed number of optional columns, as are defined in the header. This could work, but I am not sure how much it improves ...
written 9 months ago by
lh3 ♦ 30k
5
votes
9
answers
2.9k
views
9
answers
... I always think the biggest mistake in SAM (not BAM) is the integer flag because it is not human readable. I proposed letter flags, using one letter for one flag bit, but the proposal fell through. Probably I should have acted more firmly at the time. On the other hand, I am not sure how binary flag ...
written 9 months ago by
lh3 ♦ 30k
3
votes
2
answers
653
views
2
answers
... Canu uses alignments between pacbio reads to correct away most sequencing errors in raw reads. You can't do much better without using the signal information. To achieve high-quality consensus, Quiver is your only choice. ...
written 10 months ago by
lh3 ♦ 30k
5
votes
6
answers
785
views
6
answers
... Another major problem with the current long-read technologies is that they are unable to call SNPs from diploid genomes. In theory, PacBio probably can achieve the goal, but no available tools match the accuracy of Illumina data yet. Nanopore can't so far, even in theory. Without solving this proble ...
written 10 months ago by
lh3 ♦ 30k
3
votes
5
answers
1.2k
views
5
answers
... On the other bioinfo Q&A, one of the best questions is about [the difference between mathematical and bioinformatics de Bruijn graphs](https://bioinformatics.stackexchange.com/questions/146/how-to-make-a-distinction-between-the-classical-de-bruijn-graph-and-the-one-de). That question made me thi ...
written 10 months ago by
lh3 ♦ 30k
2
votes
1
answer
670
views
1
answers
... 29 is pretty low. A blat score 300 is high enough to make me worry. What is coordinate of this region? The genome build? ...
written 10 months ago by
lh3 ♦ 30k
2
votes
1
answer
670
views
1
answers
... In somatic mutation calling, multiple SNVs on one read are mostly false positives even if you see no evidence in the paired normal. I am not sure what is the exact cause, but most SNVs like these don't get validated experimentally. ...
written 10 months ago by
lh3 ♦ 30k
1
vote
11
answers
34k
views
11
answers
... Yes, as long as you use the same -s. ...
written 11 months ago by
lh3 ♦ 30k
0
votes
1
answer
1.1k
views
1
answers
... Most biologists don't run VM, let alone install a Linux in it. Your tool should simplify data processing, not complicate it. I don't know what industrial users will think about the tool, but I am moderately certain that few academia users will use it.
The dependencies you chose are too heavy. I don ...
written 11 months ago by
lh3 ♦ 30k
Latest awards to lh3
Good Answer
4 months ago,
created an answer that was upvoted at least 5 times.
For A: Why You Need Perl/Python If You Know R/Shell [Ngs Data Analysis]
Appreciated
4 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Good Answer
4 months ago,
created an answer that was upvoted at least 5 times.
For A: Indel Left/Right Alignment
Teacher
4 months ago,
created an answer with at least 3 up-votes.
For A: Next Generation Read Sequence Assembly Visualization Software
Appreciated
4 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Appreciated
5 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Teacher
7 months ago,
created an answer with at least 3 up-votes.
For A: Efficient Python data structure to store and process annotations
Appreciated
8 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Good Answer
9 months ago,
created an answer that was upvoted at least 5 times.
For A: Why You Need Perl/Python If You Know R/Shell [Ngs Data Analysis]
Appreciated
9 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Commentator
9 months ago,
created a comment with at least 3 up-votes.
For C: Remotely Access Bigwig File With Python
Appreciated
9 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Commentator
10 months ago,
created a comment with at least 3 up-votes.
For C: Remotely Access Bigwig File With Python
Epic Question
10 months ago,
created a question with more than 10,000 views.
For For Short Reads, Which Aligners Find All Hits Given Certain Edit Distance Threshold?
Scholar
10 months ago,
created an answer that has been accepted.
For A: Coordinates of the PAR3 Region on chrX and chrY
Good Answer
10 months ago,
created an answer that was upvoted at least 5 times.
For A: Why You Need Perl/Python If You Know R/Shell [Ngs Data Analysis]
Appreciated
10 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Commentator
10 months ago,
created a comment with at least 3 up-votes.
For C: Remotely Access Bigwig File With Python
Good Answer
10 months ago,
created an answer that was upvoted at least 5 times.
For A: Best Software For Detection Of Somatic Mutations From Matched Tumor:Normal Ngs D
Commentator
10 months ago,
created a comment with at least 3 up-votes.
For C: Remotely Access Bigwig File With Python
Appreciated
10 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Appreciated
11 months ago,
created a post with more than 5 votes.
For Why is academic software hard to install?
Appreciated
11 months ago,
created a post with more than 5 votes.
For Aligning Sequence Reads, Clone Sequences And Assembly Contigs With Bwa-Mem
Appreciated
11 months ago,
created a post with more than 5 votes.
For A: Why You Need Perl/Python If You Know R/Shell [Ngs Data Analysis]
Commentator
11 months ago,
created a comment with at least 3 up-votes.
For C: Remotely Access Bigwig File With Python
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 633 users visited in the last hour