User: lessismore

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lessismore890
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Posts by lessismore

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Comment: C: Technical tips to plan a resequencing project
... Dear, thanks for your very informative answer! I forgot to write that a good reference is available. Does your answer change in this case? I've seen that for example, for tea, coffee and sunflower the coverage was between x10 and x20 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895910/pdf/genes-1 ...
written 10 days ago by lessismore890
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Comment: C: Technical tips to plan a resequencing project
... I confirm a good reference is available. Thanks! ...
written 10 days ago by lessismore890
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Comment: C: Technical tips to plan a resequencing project
... Thanks for replying ! Ideally i'd like to have the highest recommended coverage to investigate SNPs and SVs in a species with a high level of heterozygosity. ...
written 13 days ago by lessismore890
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Technical tips to plan a resequencing project
... Dear all, i'm dealing with deciding the sequencing technologies to adopt for a resequencing project of 1 specific plant variety. Genome size of this species is about 700Mbp. 1. Can you suggest me desired output, preferable (mixed?) sequencing technologies to use, and, if you know that, more or ...
genome sequencing written 13 days ago by lessismore890 • updated 12 days ago by lieven.sterck8.2k
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Answer: A: How to combine multiple pheatmap figures and use common legend?
... Hi! You can use `ggplotify` and `patchwork` . Here a reproducible example. It doesn't collect the legends though.. # load libraries library(ggplot2) library(ggplotify) library(pheatmap) library(patchwork) # create random dataframe df = data.frame(matrix(rnorm(20), n ...
written 11 weeks ago by lessismore890
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Answer: A: how to connect the hub genes from PPI to the most up-regulated genes
... You can work only on the network edges then. For example extract their co-expression and protein-protein interaction networks and super-impose the PPI network to the co-expression and calculate the links and biological processes shared between them. ...
written 11 weeks ago by lessismore890
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Workflow for big gene family analysis cross-species
... Dear all, i'm dealing with a bulk of protein sequences from the same transcription factor family from distant organism families. I'd like to know what are the common good practices you use in this pipeline as i've seen that papers are very grey when they present this in their methods. My analyse ...
phylogeny gene families written 11 weeks ago by lessismore890 • updated 11 weeks ago by Jean-Karim Heriche23k
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Comment: C: Course ONLINE - RNAseq data analysis with R and Bioconductor (15-19 June)
... They use this book as learning material. ...
written 12 weeks ago by lessismore890
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Comment: A: Course ONLINE - RNAseq data analysis with R and Bioconductor (15-19 June)
... Hi! Out of curiosity, do you know where to find the solutions to the exercises of "Modern Statistics for Modern Biology"? Thanks ...
written 12 weeks ago by lessismore890
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Comment: C: Strategies to offset the effects of row-wise correction from missing data
... You can replace them with the row means. https://stackoverflow.com/questions/6918086/replace-na-values-by-row-means ...
written 12 weeks ago by lessismore890

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