User: from the mountains
from the mountains • 40
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Posts by from the mountains
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... We are looking into assembly with pacbio and illumina data, and wondering if there are any tools or well-validated workflows for hybrid assemblies, as well as any recommendations for coverage. this is for a eukaryotic genome. additionally, has anyone ever tried a pacbio-10XGenomics hybrid assembly? ...
written 5 weeks ago by
from the mountains • 40
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... I'm hoping to find a tool that will merge reads if there's a non-ambiguous overlap, and join the rest of the reads with some symbol, like a number of hyphens or NNNNs. I vaguely remember finding a tool that can do it but now I can't remember. Help is appreciated! ...
written 5 months ago by
from the mountains • 40
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... for some reason my answer posted above, i'm not sure how to move it:
`00000000 54 47 47 54 47 47 41 54 47 43 54 47 0a 3e 43 50 |TGGTGGATGCTG.>CP|`
`00000010 30 31 34 35 39 34 0a 41 43 41 47 43 43 47 41 43 |014594.ACAGCCGAC|`
`00000020 41 41 43 43 43 41 41 43 41 54 47 43 43 41 41 41 |AAC ...
written 7 months ago by
from the mountains • 40
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... `00000000 54 47 47 54 47 47 41 54 47 43 54 47 0a 3e 43 50 |TGGTGGATGCTG.>CP| `
`00000010 30 31 34 35 39 34 0a 41 43 41 47 43 43 47 41 43 |014594.ACAGCCGAC|`
`00000020 41 41 43 43 43 41 41 43 41 54 47 43 43 41 41 41 |AACCCAACATGCCAAA|`
`00000030 43 54 43 43 41 47 41 43 54 43 47 41 41 43 ...
written 7 months ago by
from the mountains • 40
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... good catch! unfortunately, it still doesn't work, in spite of both files now being ASCII text with no \r . ...
written 7 months ago by
from the mountains • 40
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... > ref.fa: ASCII text, with CRLF line terminators ...
written 7 months ago by
from the mountains • 40
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... I am getting a curious error when using bedtools to extract sequences from a reference fasta and a gff3 file.
I enter:
`/gpfs/gwngs/tools/bedtools2/bin/bedtools getfasta -s -fullHeader -fi ref.fa -bed ref.gff3`
And I get a million lines saying, for example:
> WARNING. chromosome (CP014594) w ...
written 7 months ago by
from the mountains • 40
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... thanks for your reply. I think I was misinterpreting the quast manual--i think that *after* I liftover an annotation so it corresponds to the new assembly, i could feed both the new assembly and the new annotation into quast and to report those genes plus new genes that aren't in the new annotation ...
written 7 months ago by
from the mountains • 40
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... I have generated a new fungal assembly on a species that has been assembled before, and I am trying to translate the annotations from the old assembly to the new assembly using genemark-es within quast. The quast manual says "If a gene file is provided with -G as well, both # genes in the file cover ...
written 7 months ago by
from the mountains • 40
• updated
7 months ago by
lieven.sterck • 2.6k
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... Is there any way to save the unmerged reads to separate files when using bbmerge?
I am attempting to merge reads with overlap using bbmerge, but would also like to save unmerged reads, preferably separately to R1 and R2 files.
...
written 10 months ago by
from the mountains • 40
• updated
10 months ago by
finswimmer ♦ 6.2k
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