User: k.kathirvel93

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k.kathirvel93190
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Posts by k.kathirvel93

<prev • 151 results • page 1 of 16 • next >
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how to find RT- qPCR data for perticular SEQC RNA-seq data set?
... Hi EveryOne... I have downloaded the replicates of MAQC RNA datasets from `https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE47792` IDs : GSM1156797 SEQC_ILM_BGI_A_1_L01_ATCACG_AC0AYTACXX GSM1156798 SEQC_ILM_BGI_A_1_L01_ATCACG_BC0AYPACXX GSM1156813 SEQC_ILM_BGI_A_2_L01_ACAGTG_AC0AYTACXX G ...
genome next-gen rna-seq written 6 days ago by k.kathirvel93190
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Comment: C: Volcano_plot using R
... Can you mention which tool you have used for DE? Why don't you use FDR value instead od PValue? and can you explain that how the plot shoul be ? ...
written 22 days ago by k.kathirvel93190
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Answer: C: Volcano_plot using R
... Its `log2FoldChanges` not `log2FoldChange` in your code. ...
written 22 days ago by k.kathirvel93190
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Comment: C: Parsnp Error. Why?
... Can anyOne answer/Help this ? ...
written 23 days ago by k.kathirvel93190
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Comment: C: Parsnp Error. Why?
... Hi finswimmer, I have downloaded the `.tar` package directly from the official page. ...
written 26 days ago by k.kathirvel93190
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Parsnp Error. Why?
... Hi EveryOne, I am using Parsnp to extract all the core geome SNPs from my bacterial genomes. Here is the command line `./parsnp -g ref/PAO1.gbk -d Parsnp/ -p 8 -c -o Output/`. But I got a error like this Traceback (most recent call last): File "", line 624, in NameError: name 'nam ...
software error genome next-gen snp written 26 days ago by k.kathirvel93190
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Answer: A: Can logCPM in edgeR be used to compare expression of genes ?
... Better do not go with only logFC, consider the genes which have high normalized counts and difference compared to control, with logFC <2 or 1.5. ...
written 8 weeks ago by k.kathirvel93190
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Comment: C: How can I extract SNP from bacterial genome?
... https://www.ebi.ac.uk/sites/ebi.ac.uk/files/content.ebi.ac.uk/materials/2014/140217_AgriOmics/dan_bolser_snp_calling.pdf Is this protocol will help for *p.aeruginosa* also ? ...
written 8 weeks ago by k.kathirvel93190
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How can I extract SNP from bacterial genome?
... HI EveryOne, I Have a in-house *pseudomonas aeruginosa* bacterial whole genome data, and now i want to extract SNP from that. How can i do that ? Thanks in advance for any suggestions. ...
gene genome next-gen snp sequencing written 8 weeks ago by k.kathirvel93190 • updated 8 weeks ago by shubhra.bhattacharya120
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Comment: C: target gene selection for miRNA
... "I have a list of differentially expressed genes" - genes or miRNAs ? ...
written 8 weeks ago by k.kathirvel93190

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