User: Picasa

gravatar for Picasa
Picasa390
Reputation:
390
Status:
Trusted
Location:
Last seen:
3 days, 10 hours ago
Joined:
2 years, 11 months ago
Email:
p*********@gmail.com

Posts by Picasa

<prev • 262 results • page 1 of 27 • next >
0
votes
0
answers
84
views
0
answers
Comment: C: What coverage is recommended for amplicon sequencing?
... Oh sorry, I will detail more. 50x or 100x seems quite a lot no ? I have 7 genes and 200 individual of the same species (but different populations), so a total of 1400 genes to anaylse. The genes are quite short (500 bp in average). And my goal is to make a tree of genes. ...
written 3 days ago by Picasa390
0
votes
0
answers
84
views
0
answers
Comment: C: What coverage is recommended for amplicon sequencing?
... Yes I need a representative sequence for each gene and species but my idea was more like sequencing each gene for each species at a certain coverage (how much, is my question) with high-throughput sequencing and get the "most represented". Example for one gene of one species, if I have a coverage o ...
written 3 days ago by Picasa390
1
vote
0
answers
84
views
0
answers
What coverage is recommended for amplicon sequencing?
... Hello, My goal is to make a gene tree of different genes and multiple species. I will amplify genes via pcr and get amplicon sequencing. Do you have any recommendation for the sequencing coverage ? Thanks. ...
amplicon coverage written 4 days ago by Picasa390
0
votes
2
answers
185
views
2
answers
Comment: C: How to extract the same mapped region by multiple samples ?
... but it is already in bed format no ? ...
written 7 weeks ago by Picasa390
0
votes
2
answers
185
views
2
answers
Comment: C: How to extract the same mapped region by multiple samples ?
... Thanks Pierre, your command was very useful. I am wondering if you know a way to extract regions of these "comm.bed" but relative to my 3 samples (S1.bam S2.bam S3.bam) So for instance : RF01 10 3295 is the coordinates in the reference genome but I would like the coordinates in my sampl ...
written 7 weeks ago by Picasa390
0
votes
0
answers
127
views
0
answers
Comment: C: Primer-blast impossible with multiple sequences ?
... Thanks but it is not what I am looking for. This thread talks about finding DNA sequences from a primer pair, while I want the reverse (finding primer pair from dna sequence) ...
written 9 weeks ago by Picasa390
0
votes
0
answers
127
views
0
answers
Primer-blast impossible with multiple sequences ?
... Hi Biostars, I am trying to design primers. For this I have multiple sequences in a fasta file and I have found this program that give kind of nice visualisation: https://www.ncbi.nlm.nih.gov/tools/primer-blast/ 1) However I cannot paste multiples sequences .. Is it normal or I am doing something ...
sequences primer-blast written 9 weeks ago by Picasa390 • updated 7 weeks ago by Biostar ♦♦ 20
0
votes
2
answers
185
views
2
answers
Comment: C: How to extract the same mapped region by multiple samples ?
... Thanks, but Pierre method is what I was searching for. Your method output regions where at least 2 samples mapped while I wanted all the samples ...
written 9 weeks ago by Picasa390
0
votes
2
answers
185
views
2
answers
Comment: C: How to extract the same mapped region by multiple samples ?
... Thanks Pierre, can you explain this ? int($2)-1 ...
written 9 weeks ago by Picasa390
0
votes
2
answers
185
views
2
answers
Comment: C: How to extract the same mapped region by multiple samples ?
... Yes there is one bam per sample. My idea is to merge them with samtools and then use a program (? this is what I called for help) that extract the regions that have been mapped in common between the 3 samples. ...
written 10 weeks ago by Picasa390

Latest awards to Picasa

Popular Question 5 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 6 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 weeks ago, created a question with more than 1,000 views. For What is the best way to compute scaffold coverage ?
Great Question 8 weeks ago, created a question with more than 5,000 views. For LD pruning and other QC before association analysis ?
Popular Question 9 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 9 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 12 weeks ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 4 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 4 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 5 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 6 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 6 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 6 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 7 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 8 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 9 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 9 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 10 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file
Popular Question 10 months ago, created a question with more than 1,000 views. For What is the best way to compute scaffold coverage ?
Popular Question 10 months ago, created a question with more than 1,000 views. For Coverage per contig from a BAM file

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1172 users visited in the last hour