User: Picasa
Picasa • 300
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Posts by Picasa
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... Hi,
I have successfully annotated my genome with MAKER. Now I have a gff file that I want to post process /filter.
In particular, I would like to discard gene that is below a certain AED score.
1) Is there an AED treshold from where a gene is not strongly supported ? if yes, do you have some refe ...
written 4 weeks ago by
Picasa • 300
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... I have the same error even without using the -s in cuffmerge ... any solution ? ...
written 10 weeks ago by
Picasa • 300
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... Well Maker needs (not mandatory but recommended) transcriptome to infer gene with gene predictor.
...
written 11 weeks ago by
Picasa • 300
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... yes, and i want to provide rna seq evidence ...
written 11 weeks ago by
Picasa • 300
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... Hi,
I would like to provide rna seq evidence to Maker for genome annotation.
For the transcriptome building, I have done a reference based assembly with STAR+cufflinks but I am not sure if it is the correct way,
Do you have some experience with this ? ...
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... Hi, I like your explanation, do you have papers that describe this possibility of chimeric contigs ? ...
written 3 months ago by
Picasa • 300
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... Hi Roxane,
I was reading this for quiver
https://github.com/PacificBiosciences/GenomicConsensus
and I would like to know if you have some benchmark using the different algorithm proposed by variantCaller (quiver,arrow,plurality,poa,best).
I mean, does it change a lot ? The link above said that ...
written 3 months ago by
Picasa • 300
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... Maybe I was not clear with my question.
I am not asking which assembler is the best, but I am looking just for papers with different project that have done a de novo genome assembly and had good results.
(like the panda genome)
http://www.nature.com/nature/journal/v463/n7279/full/nature08696.htm ...
written 4 months ago by
Picasa • 300
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... Hi,
I am looking for papers of the best de novo assemblies ever done with (and only) Illumina data for differents kind of organisms (bacteria, insect, mammals, plants etc.)
By best I mean accurate, not fragmented (high N50), no contaminations, good completeness scores etc.
Do you know any ?
Than ...
written 4 months ago by
Picasa • 300
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... I have the sequencing coverage information; but why is it important ? ...
written 5 months ago by
Picasa • 300
Latest awards to Picasa
Popular Question
5 months ago,
created a question with more than 1,000 views.
For Best strategy for de novo assembly with illumina reads ?
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6 months ago,
created a question with more than 1,000 views.
For Best strategy for de novo assembly with illumina reads ?
Centurion
10 months ago,
created 100 posts.
Student
11 months ago,
asked a question with at least 3 up-votes.
For Best strategy for de novo assembly with illumina reads ?
Supporter
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voted at least 25 times.
Scholar
13 months ago,
created an answer that has been accepted.
For A: 16S Paired-End Illumina MiSeq Data in Qiime
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14 months ago,
created 50 posts within first three months of joining.
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