User: Picasa
Picasa • 390
- Reputation:
- 390
- Status:
- Trusted
- Location:
- Last seen:
- 3 days, 10 hours ago
- Joined:
- 2 years, 11 months ago
- Email:
- p*********@gmail.com
Posts by Picasa
<prev
• 262 results •
page 1 of 27 •
next >
0
votes
0
answers
84
views
0
answers
... Oh sorry, I will detail more. 50x or 100x seems quite a lot no ?
I have 7 genes and 200 individual of the same species (but different populations), so a total of 1400 genes to anaylse.
The genes are quite short (500 bp in average).
And my goal is to make a tree of genes.
...
written 3 days ago by
Picasa • 390
0
votes
0
answers
84
views
0
answers
... Yes I need a representative sequence for each gene and species but my idea was more like sequencing each gene for each species at a certain coverage (how much, is my question) with high-throughput sequencing and get the "most represented".
Example for one gene of one species, if I have a coverage o ...
written 3 days ago by
Picasa • 390
1
vote
0
answers
84
views
0
answers
... Hello,
My goal is to make a gene tree of different genes and multiple species.
I will amplify genes via pcr and get amplicon sequencing.
Do you have any recommendation for the sequencing coverage ?
Thanks. ...
written 4 days ago by
Picasa • 390
0
votes
2
answers
185
views
2
answers
... but it is already in bed format no ? ...
written 7 weeks ago by
Picasa • 390
0
votes
2
answers
185
views
2
answers
... Thanks Pierre, your command was very useful.
I am wondering if you know a way to extract regions of these "comm.bed" but relative to my 3 samples (S1.bam S2.bam S3.bam)
So for instance :
RF01 10 3295
is the coordinates in the reference genome
but I would like the coordinates in my sampl ...
written 7 weeks ago by
Picasa • 390
0
votes
0
answers
127
views
0
answers
... Thanks but it is not what I am looking for. This thread talks about finding DNA sequences from a primer pair, while I want the reverse (finding primer pair from dna sequence) ...
written 9 weeks ago by
Picasa • 390
0
votes
0
answers
127
views
0
answers
... Hi Biostars,
I am trying to design primers. For this I have multiple sequences in a fasta file and I have found this program that give kind of nice visualisation:
https://www.ncbi.nlm.nih.gov/tools/primer-blast/
1) However I cannot paste multiples sequences .. Is it normal or I am doing something ...
0
votes
2
answers
185
views
2
answers
... Thanks, but Pierre method is what I was searching for.
Your method output regions where at least 2 samples mapped while I wanted all the samples ...
written 9 weeks ago by
Picasa • 390
0
votes
2
answers
185
views
2
answers
... Thanks Pierre, can you explain this ?
int($2)-1 ...
written 9 weeks ago by
Picasa • 390
0
votes
2
answers
185
views
2
answers
... Yes there is one bam per sample.
My idea is to merge them with samtools and then use a program (? this is what I called for help) that extract the regions that have been mapped in common between the 3 samples. ...
written 10 weeks ago by
Picasa • 390
Latest awards to Picasa
Popular Question
5 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
6 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 weeks ago,
created a question with more than 1,000 views.
For What is the best way to compute scaffold coverage ?
Great Question
8 weeks ago,
created a question with more than 5,000 views.
For LD pruning and other QC before association analysis ?
Popular Question
9 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
9 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
12 weeks ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
4 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
4 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
5 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
6 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
6 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
6 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
8 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
9 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
9 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
10 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Popular Question
10 months ago,
created a question with more than 1,000 views.
For What is the best way to compute scaffold coverage ?
Popular Question
10 months ago,
created a question with more than 1,000 views.
For Coverage per contig from a BAM file
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 1172 users visited in the last hour