User: Varun Gupta

gravatar for Varun Gupta
Varun Gupta1.0k
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Posts by Varun Gupta

<prev • 218 results • page 1 of 22 • next >
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Filtering reads on the basis of percentage of ambiguous(N's) characters
... Hi, I want to filter my fastq reads based on ambiguous characters, basically **N's**. So if I have a read sequence having N's greater than 5%, I want to discard that read, if lower than 5%, I want to keep it. It would be really helpful if someone know about any tool which already does that. Thanks ...
filter fastq rna-seq written 8 weeks ago by Varun Gupta1.0k • updated 8 weeks ago by Pierre Lindenbaum111k
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Comment: C: Picard Mark Duplicates
... Does picard takes soft clipped bases into account then? ...
written 4 months ago by Varun Gupta1.0k
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Comment: C: Picard Mark Duplicates
... Hi finswimmer, I tried the same thing with samtools rmdup and it considers them as duplicates and retains the one with high mapping quality. Regarding the soft clip bases, I don't think soft clipped bases has to do with this thing because the position 88015806 in the above example is the position st ...
written 4 months ago by Varun Gupta1.0k
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Comment: C: Picard Mark Duplicates
... Thanks Pierre. Can we provide a list of Barcodes and not 1 BARCODE sequence to BARCODE_TAG option? The library was indeed created with Unique Molecular Barcodes(96 of them) ...
written 4 months ago by Varun Gupta1.0k
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Picard Mark Duplicates
... Hi, I have used Picard MarkDuplicates tool to remove duplicates from my bam file. Understanding it correctly, if there are more than 1 reads starting at the same position, then after removing for duplicates, only 1 read will be present in the bam file generated from MARKDUPLICATES based on its qual ...
picard markduplicates written 4 months ago by Varun Gupta1.0k • updated 11 weeks ago by marcela.uliano40
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Comment: C: genomeCoverageBed for per nucleotide coverage
... yup, works fine, must say better than genomeCoverageBed because in genomeCoverageBed, if you have all the headers(having all the chromosomes) in the bam file and only a particular chr alignments you want, it still goes through all the chromosomes. ...
written 4 months ago by Varun Gupta1.0k
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Comment: C: genomeCoverageBed for per nucleotide coverage
... Hi Kevin, Thanks for your reply. I looked at the bedtools manual for coverage function. If I want per nucleotide coverage for the bed file(as is my case), the command should be like this bedtools coverage -a mygenes.bed -b test.bam -d Then this is the output chr5 10000 23456 10001 14 ...
written 4 months ago by Varun Gupta1.0k
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genomeCoverageBed for per nucleotide coverage
... Hello, I want to get per nucleotide coverage for my particular gene (geneX) which I added in the fasta file. There are other chromosomes present in the bam file, whose per nu coverage I am not interested in. When I use genomeCoverageBed tool to calculate per nucleotide coverage for my gene I also g ...
bedtools written 4 months ago by Varun Gupta1.0k • updated 4 months ago by Kevin Blighe25k
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Comment: C: Saving IGV images for bigwig files
... ![enter image description here][1]Hi Venu, I tried making plots by providing this command ./pyGenomeTracks --tracks tracks.ini --BED ~/test.bed -out fig_ The graph is showing too much upstream and downstream of my bed coordinates. Any way to fix that Also how can i display the name of the feat ...
written 6 months ago by Varun Gupta1.0k
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Comment: C: Saving IGV images for bigwig files
... It got installed here /home/varun/.local/bin ...
written 6 months ago by Varun Gupta1.0k

Latest awards to Varun Gupta

Popular Question 17 days ago, created a question with more than 1,000 views. For Removing low quality ends from a fastq file
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Popular Question 4 months ago, created a question with more than 1,000 views. For Removing low quality ends from a fastq file
Student 5 months ago, asked a question with at least 3 up-votes. For Read Counts In Intronic Region
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Teacher 7 months ago, created an answer with at least 3 up-votes. For A: Help With Perl Script: Store Fasta Sequences Into A Hash.
Popular Question 7 months ago, created a question with more than 1,000 views. For RNA Seq datasets for different tissues not Poly-A selected
Popular Question 11 months ago, created a question with more than 1,000 views. For RNA Seq datasets for different tissues not Poly-A selected
Teacher 11 months ago, created an answer with at least 3 up-votes. For A: Help With Perl Script: Store Fasta Sequences Into A Hash.
Popular Question 11 months ago, created a question with more than 1,000 views. For Yeast genome SK1 strain
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Appreciated 13 months ago, created a post with more than 5 votes. For A: Help With Perl Script: Store Fasta Sequences Into A Hash.
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Guru 13 months ago, received more than 100 upvotes.
Popular Question 14 months ago, created a question with more than 1,000 views. For Homologous Genes In Mouse For Given Human Genes
Popular Question 15 months ago, created a question with more than 1,000 views. For Homologous Genes In Mouse For Given Human Genes
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Epic Question 16 months ago, created a question with more than 10,000 views. For Bwa Mem -M Option

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