... I was reading a paper and it seems that they compare whole genome pool-sequencing with other technique saying that the the cost of reduced representation sequencing technique by reducing the amount of DNA to be sequenced.
> In contrast to the whole-genome approach of Pool-seq, the cost savings
... From what I understood, [allele frequency spectrum] is everything that concerns the frequency of all of the alleles studied in a population. It can be estimated with Tajima's D, theta, etc.
But how is it different from the *joint* allele frequency spectrum?
: https://en.wikipedia.org/wi ...
... I tried to find a paper showing how to do this, but I found nothing about ˙øw to create a genetic pedigree using neutral markers. Is there a way to construct a pedigree using RAD data?
Most of the information I found is talking about *adding* a PED file for analysis, but not how to generate a pedi ...
... In an article about Tibetan adaptation, they talk about a population branch statistic.
> By comparing the three pairwise FST values between these three
> samples, we can estimate the frequency change that occurred in the
> Tibetan popu ...
... In our lab, we are using ddRAD sequencing with DBR to deal with PCR duplicates. We basically add a DBR, in the Read2 paired end. Here is an example, (the DBR is square brackets):
... In the [Adapterama paper], they are talking about a way to tag individuals within a library preparation for sequencing.
At some point, they explain their primer and show this:
` iTru5_01_A: 5';AATGATACGGCGACCACCGAGATCTACAC tag;ACCGACAA 3';ACACTCTTTCCCTA*C`
What is the star for in this ...