User: Rahul Sharma

gravatar for Rahul Sharma
Rahul Sharma600
Reputation:
600
Status:
Trusted
Location:
Germany
Website:
http://www.bik-f.de/ro...
Scholar ID:
Google Scholar Page
Last seen:
6 hours ago
Joined:
8 years, 5 months ago
Email:
r***********@gmail.com

Posts by Rahul Sharma

<prev • 85 results • page 1 of 9 • next >
1
vote
1
answer
329
views
1
answers
Answer: A: Establishing a metagenomics lab/hardware requirements
... That largely depends on type of analysis one wants to perform. For de-novo assemblies based on k-mer algorithms requires much RAM (for microbial genomes of size upto 200 Mbs my jobs consumed upto 512 Gbs of RAM). For routine bioinformatics analysis 124Gbs of RAM should be fine. For much computationa ...
written 11 months ago by Rahul Sharma600
2
votes
2
answers
270
views
2
answers
Answer: A: Are there institutions/companies which provide paid access to computational reso
... Thanks guys for the helpful comments. Here are the services I could short-list along with the pricing information: Google Compute: https://cloud.google.com/compute/pricing?authuser=1 Microsoft Azure: https://azure.microsoft.com/en-us/pricing/details/virtual-machines/linux/ Amazon AWS: https://aws ...
written 13 months ago by Rahul Sharma600
0
votes
0
answers
266
views
0
answers
Tools to access variation of ploidy at certain genomic loci using WES
... Hi all, I am checking for ploidy level variations after endomitosis among diseased and control samples using WES data at certain chromosome loci. I could not find any specific tool for such an analysis. There are tools like nQuire and ploidyNGS for checking the ploidy levels on whole genomes. Would ...
endomitosis wes ploidy written 14 months ago by Rahul Sharma600
4
votes
2
answers
270
views
2
answers
Are there institutions/companies which provide paid access to computational resources?
... Hi all, Mammalian whole Genome/Exome Sequencing data analysis in particular de-novo assembly based projects usually require >512 Gbs or even >1TB RAM. I get such dataset twice or thrice a year. Instead of buying a computational machine with this much of RAM will cost >10,000 Euros. Are the ...
0
votes
0
answers
476
views
0
answers
Job: Bioinformatician - Genome Biologics - Accelerating Drug Development
... **Position type:** Permanent, 100%, based in Frankfurt (DE). Genome Biologics is a Biotherapeutics company headquartered in Germany with a strong background and proven track record in silico drug screening, in vivo preclinical drug testing and state-of-the-art transgenic technologies. **Job descrip ...
next-gen python job machine learning R rna-seq written 14 months ago by Rahul Sharma600
0
votes
1
answer
634
views
1
answers
Answer: A: Freelance bioinformatics jobs
... This company:"https://www.aomics.com/" could recommend you someone with affordable price and a quality work. ...
written 17 months ago by Rahul Sharma600
1
vote
0
answers
833
views
0
answers
Comment: C: 25 DE genes with 8 samples and 4 DE genes with 16 samples
... Which software are you using for finding DEGs? Maybe it would be helpful to compare different software outputs. How these samples are placed in a PCA plot? I would also look into the read count for individual replicates and see how the read counts are different in each replicate of the 4 DEGs. I use ...
written 21 months ago by Rahul Sharma600
1
vote
2
answers
917
views
2
answers
Ribo-seq couts are more than RNA-seq count
... I am analysing Ribo-Seq data (Illumina single end ~25 bp reads) using the Xtail pipeline. I also have RNA-Seq data sequenced using the same samples and sequencing chemistry (Illumina single end ~25 bp reads). During the bioinformatic analyses, we figured out that in around 48% of genes the Ribo-seq ...
ribo-seq ngs data analysis xtail rna-seq written 2.2 years ago by Rahul Sharma600 • updated 21 months ago by afeizi20
1
vote
1
answer
4.2k
views
1
answers
Answer: A: Formula to calculate FPKM
... Although I would use DESeq2, Cuffdiff or other published and well established methods for finding DEGs. Here is a link where they explained the formula in details: [RPKM, FPKM and TPM, clearly explained][1] [1]: http://www.rna-seqblog.com/rpkm-fpkm-and-tpm-clearly-explained/ ...
written 2.3 years ago by Rahul Sharma600
0
votes
3
answers
691
views
3
answers
Answer: A: gene expression database for quary a gene
... Although that is very tricky to get gene signatures that could match to your query signatures, as many factor influence the expression profiles e.g cell type used, controls, number of replicates and way of generating expression profiles. Here are few on-line resources that I use for my analyses: ht ...
written 2.4 years ago by Rahul Sharma600

Latest awards to Rahul Sharma

Popular Question 6 weeks ago, created a question with more than 1,000 views. For MIRA for metagenome assembly
Great Question 5 months ago, created a question with more than 5,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Epic Question 7 months ago, created a question with more than 10,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Scholar 13 months ago, created an answer that has been accepted. For A: gene ortholog conversion
Epic Question 2.2 years ago, created a question with more than 10,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Scholar 2.4 years ago, created an answer that has been accepted. For A: gene ortholog conversion
Popular Question 2.5 years ago, created a question with more than 1,000 views. For MIRA for metagenome assembly
Popular Question 2.6 years ago, created a question with more than 1,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Great Question 2.8 years ago, created a question with more than 5,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Appreciated 3.6 years ago, created a post with more than 5 votes. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Good Question 3.6 years ago, asked a question that was upvoted at least 5 times. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Popular Question 3.8 years ago, created a question with more than 1,000 views. For Does Velvet Assembler Use Ram Of All Nodes Altogether?
Teacher 3.8 years ago, created an answer with at least 3 up-votes. For A: Download All The Bacterial Genomes From Ncbi
Great Question 4.0 years ago, created a question with more than 5,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Teacher 4.2 years ago, created an answer with at least 3 up-votes. For A: Download All The Bacterial Genomes From Ncbi
Great Question 4.2 years ago, created a question with more than 5,000 views. For How To Extract Cds And Protein Sequences From Cufflinks Transcripts.Gtf File?
Popular Question 4.7 years ago, created a question with more than 1,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?
Teacher 4.9 years ago, created an answer with at least 3 up-votes. For A: Denovo Assembly Of Paired And Mate Paired Reads
Popular Question 5.1 years ago, created a question with more than 1,000 views. For How To Extract Reads-Pairs Aligned Concordantly Exactly 1 Time?

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1697 users visited in the last hour