User: corend

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corend70
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Posts by corend

<prev • 52 results • page 1 of 6 • next >
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Comment: C: bedtools intersect with match on name column
... My bad, my example was not a MWE, I will validate your answer. If you replace 10 25 by 10 35 here you will see the problem. ...
written 16 days ago by corend70
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Answer: A: bedtools intersect with match on name column
... I finaly found out how to do it: As I want a Chromosome and name match, I just make a bedfile in the format `chr+name start end name`, and it works well. ...
written 16 days ago by corend70
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Comment: C: bedtools intersect with match on name column
... Sorry, it is not working, here is the problem: In the second step, you don't intersect line by line, so the problem is still the same. Just try it and you will see. ...
written 16 days ago by corend70
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bedtools intersect with match on name column
... I have files `a.bed` and `b.bed` `a.bed` chr1 10 20 geneA chr1 30 40 geneB `b.bed` chr1 5 15 geneA chr1 10 25 geneB if I `intersectbed` those files I will obtain: chr1 10 15 geneA chr1 10 20 geneA But I would like the `name` column to match between files so that i wil ...
bedtools intersectbed written 17 days ago by corend70
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Comment: C: What is the ideal FDR and fold change cutoff to consider in a RNA-seq experiment
... You should definitely use a threshold on both metrics. Depends on how many DEGs you want to retrieve, take a look at the litterature, which thresholds are used in other papers. Then look how many DEGs you have with those thresholds and modify if necessary. Also you should consider for the Fold ch ...
written 8 weeks ago by corend70
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Comment: C: Mapping to repeat consensus sequences?
... I am not a ChIP seq specialist. But my personal point of view would be to do the peak calling, and then compare to the repeatmasker gff. ...
written 9 weeks ago by corend70
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Comment: C: Mapping to repeat consensus sequences?
... Repeat modeler finds the repeated sequences in the genome. It gives them a number (your x), and compares them to repbase. If the sequence has a hit, it is annotated with the hit in repbase. > How can I obtain consensus sequences for LTRs or specific families of LTRs? The consensi.fa file alread ...
written 9 weeks ago by corend70
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Answer: C: isolate the output of lignment in fastq format
... https://www.biostars.org/p/770/ ...
written 9 weeks ago by corend70
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Comment: C: Detection Transposable Elements in eukaryote
... Is it repeat modeler or repeat masker that do not find them? Repeat modeler only finds what is repeated. If a LTR is old and present in only one copy, it might remain undetected by repeat modeler. Then you should use LTRharvest that uses the structure of the LTR to detect it and not it's repeated na ...
written 9 weeks ago by corend70
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Comment: C: Why are GC% per base important in quality control of reads?
... Looks like it is just biased because of the read length. After 24bp the number of read is decreasing, as the GC%. ...
written 9 weeks ago by corend70

Latest awards to corend

Scholar 16 days ago, created an answer that has been accepted. For A: bedtools intersect with match on name column
Supporter 12 months ago, voted at least 25 times.

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