User: mohammedtoufiq91
mohammedtoufiq91 • 40
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Posts by mohammedtoufiq91
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... Hi,
I am interested to integrate Metagenomics and transcriptomics (qRT-PCR) data. I would like to know if there are any tools to perform the integrated analysis using Metagenomics and transcriptomics results or any resources would be helpful.
Best Regards,
Toufiq ...
written 4 days ago by
mohammedtoufiq91 • 40
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... Hi,
I have a question on qRT-PCR data analysis. The gene panel has more than 200 genes along with the multiple reference/housekeeping genes. I have 50 paired samples (i.e Before vs After Vitamin supplementation) and 6 pooled controls (3 Pooled controls in one plate and 3 in another plate as interpl ...
written 4 days ago by
mohammedtoufiq91 • 40
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... Hi,
When I plot the hierarchical clustering heatmap using the significant genes (-DCt) between two conditions before vs after, I observe opposite profiles i.e Before (Red-max) and After (Green-min). However, when I run hierarchical clustering on an ANOVA list created from DCt, the profiles for the ...
written 7 weeks ago by
mohammedtoufiq91 • 40
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... Hi,
I am using geNorm, Normfinder, RefFinder, and BestKeeper to identify and select the appropriate reference genes for the normalization in the RT-qPCR analysis. I was trying to identify the tools to calculate the pairwise variations (Vn/n + 1) to determine the optimal number of reference genes to ...
written 10 weeks ago by
mohammedtoufiq91 • 40
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... Thank you. This was helpful. ...
written 10 weeks ago by
mohammedtoufiq91 • 40
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... Hi,
I have a normalized data file containing gene symbols as first column followed by various samples (normalized data). I was looking to extract the Ensembl IDs corresponding to the gene symbols. Basically, I would like to have both gene symbols and Ensembl IDs in the data file for further downstr ...
written 11 weeks ago by
mohammedtoufiq91 • 40
• updated
11 weeks ago by
Erin_Ensembl • 370
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Comment:
C: Tools for qRT-PCR data analysis
... Thank you for the inputs. ...
written 3 months ago by
mohammedtoufiq91 • 40
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... Thank you. This was helpful. ...
written 3 months ago by
mohammedtoufiq91 • 40
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... Hi,
I made use of some of the packages for the analysis of the RNA-Seq data starting from the alignment till the expression analysis. This was just for some of the samples from my large datasets, however, when applying these packages to my large datasets it would be tedious and time consuming, and ...
written 3 months ago by
mohammedtoufiq91 • 40
• updated
3 months ago by
WouterDeCoster ♦ 39k
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... Hi,
I have the qRT-PCR data and I would like to analyse this data from the Normalisation till the downstream analysis. Basically I would like to perform the calculation of DCt, DDCt, and 2^(-DDCt) including some of the plots. Could you kindly assist me with this.
Thank you,
Toufiq ...
written 3 months ago by
mohammedtoufiq91 • 40
• updated
3 months ago by
l.souza • 70
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