User: elb

gravatar for elb
elb110
Reputation:
110
Status:
Trusted
Location:
Torino
Last seen:
2 days, 12 hours ago
Joined:
6 years, 2 months ago
Email:
l****************@gmail.com

about me

Posts by elb

<prev • 40 results • page 1 of 4 • next >
0
votes
1
answer
51
views
1
answer
Illumina bead chip data normalisation
... Hi guys, could you suggest me a way to normalize Illumina expression beadchip data? For example, starting from the `GSE32025_non-normalized.txt.gz` file, could you suggest me some few steps (preferentially in R) to finally obtain a normalized data matrix as for Affymetrix RMA normalisation? I sup ...
illumina gene expression limma written 2 days ago by elb110 • updated 2 days ago by Kevin Blighe28k
4
votes
1
answer
67
views
1
answer
Cross platform gene expression comparison and threshold choice
... Hi guys, I have a question about how to choose a reasonable threshold to define genes to be expressed (not differentially expressed but simply expressed) to compare gene expression measurements from different platforms. In other words I have to check if my list of genes is expressed using an RNA se ...
gene expression microarrays rna-seq written 5 days ago by elb110 • updated 5 days ago by Devon Ryan84k
0
votes
0
answers
235
views
0
answers
Comment: C: FeatureCounts on HG38
... Successfully assigned reads is different ...
written 9 weeks ago by elb110
4
votes
0
answers
235
views
7 follow
0
answers
FeatureCounts on HG38
... Hi guys, I'm trying to move from HG19 to HG38. While running FeatureCounts using Refseq*.gtf I see some differences between HG19 and HG38. This I suppose is expected but I only would like to know if I'm doing wrong since I'm a newbie in the RNA-Seq HG38 analysis. Here some outputs of FeatureCounts ...
refseq star rna-seq hg19 written 9 weeks ago by elb110 • updated 9 weeks ago by h.mon19k
0
votes
1
answer
197
views
1
answers
Comment: C: Merge .bam files by groups of lanes
... It is fantastic! It works perfectly. Thank you very very much! ...
written 9 weeks ago by elb110
7
votes
1
answer
197
views
6 follow
1
answer
Merge .bam files by groups of lanes
... Hi guys, I have a folder with around 300 `.bam` files. Each `.bam` file is a lane of a sample and hence 4 lanes make a sample. I would like to merge the .bam files of the four lanes in a single one by `_*S*_` where S is followed by a number that represent the number of the sample (e.g. `my_experime ...
bam samtools rna-seq written 9 weeks ago by elb110 • updated 9 weeks ago by WouterDeCoster32k
0
votes
1
answer
208
views
1
answers
Comment: C: RNA-Seq error in htseq-count
... So basically I'm doing some naive mistakes...I try to run properly. Thank you very much. ...
written 10 weeks ago by elb110
2
votes
1
answer
208
views
1
answer
RNA-Seq error in htseq-count
... Hi guys, I really do not know what is happening to my RNA seq data analysis pipeline and hence I'm looking for some help. Although I found some related posts and I tried to follow instructions to solve the problem I'm still here and I'm getting crazy. Briefly: the steps are the following: # STA ...
next-gen rna-seq sequencing written 10 weeks ago by elb110 • updated 10 weeks ago by swbarnes24.0k
0
votes
0
answers
232
views
0
answers
Comment: C: Network inference analysis with small sample size
... I have no idea...I used the mutual information in the past but with 300 samples... ...
written 5 months ago by elb110
1
vote
0
answers
232
views
0
answers
Network inference analysis with small sample size
... Guys, I have 5 samples (patients) and around 12.000 expressed genes. I would like to predict some interactions (networks) among that genes in terms of gene expression in each sample and across the samples. My question is: due to the small sample size, is there some statistic or some algorithm for t ...
sample size network gene expression written 5 months ago by elb110 • updated 5 months ago by genomax55k

Latest awards to elb

Supporter 10 weeks ago, voted at least 25 times.
Great Question 4 months ago, created a question with more than 5,000 views. For Check For Co-Expressed Genes In Microarray Experiments
Popular Question 6 months ago, created a question with more than 1,000 views. For regulatory regions discovery and alignment
Popular Question 6 months ago, created a question with more than 1,000 views. For Transcription Factors Annotation
Great Question 3.4 years ago, created a question with more than 5,000 views. For Check For Co-Expressed Genes In Microarray Experiments
Popular Question 3.4 years ago, created a question with more than 1,000 views. For Transcription Factors Annotation
Student 3.4 years ago, asked a question with at least 3 up-votes. For Looking For Network Visualization Tools
Popular Question 5.8 years ago, created a question with more than 1,000 views. For Check For Co-Expressed Genes In Microarray Experiments
Popular Question 5.8 years ago, created a question with more than 1,000 views. For Fold Change Detection In Affymetrix Microarray Data Without Replicates
Popular Question 5.8 years ago, created a question with more than 1,000 views. For Looking For Network Visualization Tools
Student 5.8 years ago, asked a question with at least 3 up-votes. For Looking For Network Visualization Tools

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 756 users visited in the last hour