User: kanwarjag

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kanwarjag1.1k
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Posts by kanwarjag

<prev • 380 results • page 1 of 38 • next >
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Comment: A: SCRNA-seq experiment reads
... What should be the easiest way to remove all N from 3' ...
written 10 weeks ago by kanwarjag1.1k
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SCRNA-seq experiment reads
... I have ScRNA-seq data for version3 prep of 10X, R2 has several Ns to the end and few seq look like as follow- NAATTACATAAATTAGCCGTGCATCGTTGCGGGTGCCTGTAATCCCAGCTACTCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NCTCTAAACAACATCCCAGAGATGGCTGGGCGCGGTGGCTCACACCTGTAATCCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNN ...
rna-seq written 10 weeks ago by kanwarjag1.1k • updated 10 weeks ago by lieven.sterck9.4k
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extracting count matrix from default cell ranger files
... I am looking for a non programmatic method or additional software that can make count matrix of genes from three file: barcode.tsv feature.tsv matrix.mtx Is there any tool or software? Thanks ...
rna-seq written 12 weeks ago by kanwarjag1.1k • updated 12 weeks ago by ATpoint44k
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merging bam from two samples
... Can I merge two bams from different samples (biological replicates) using simply cat command? ...
alignment written 3 months ago by kanwarjag1.1k • updated 3 months ago by GenoMax94k
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Comment: C: sparse peak calling
... The low read depths and background levels and broader peaks. ...
written 3 months ago by kanwarjag1.1k
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sparse peak calling
... I have a Chipseq data and I expects peaks should be sparse. Which tool may be best to call sparse peaks? Can I also use same tool for calling sparse peaks of cut and Run experiment. Thanks ...
chip-seq written 3 months ago by kanwarjag1.1k • updated 3 months ago by AS0
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SCRNA-seq experiment reads
... I am planning a 10X single cell RNAseq library preparation and sequencing in Nova seq. For human genome to look at standard differential expression and cell types, how many reads are required / sample. Is there any standard (not rare) rule of thumb how many cells should be sequenced. I have read 10 ...
rna-seq written 3 months ago by kanwarjag1.1k
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aligning 5' seq with - white list file
... I want to align 10x sequencing file with whitelist of barcode for quality checks. The reason I want to do is as running ranger or Starsolo does not extract any bar code. What should be the best approach to extract 28 bp from Fastq and find the part of sequence in whitelist? Thanks ...
alignment written 3 months ago by kanwarjag1.1k • updated 3 months ago by Kevin Blighe69k
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GTF/GFF3 file compatible with (GRCh38/hg38)(hg38)
... I am using Reference genome -Human Dec2013 (GRCh38/hg38)(hg38) and gene model Homo_sapiens.GRCh38.101.gff3.gz however I get error saying chromosome naming is not matching. I know that Ensembel uses Chr as prefix. The question is if I use Human Dec2013 (GRCh38/hg38)(hg38) as reference assembly, which ...
rna-seq written 3 months ago by kanwarjag1.1k
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Comment: C: STARsolo config for 10x Chromium v1, v2, v3
... Unfortunately https://singlecell.usegalaxy.eu/ it will not let me fine tune such parameters. ...
written 3 months ago by kanwarjag1.1k

Latest awards to kanwarjag

Great Question 4 months ago, created a question with more than 5,000 views. For GRCH38 and hg19
Epic Question 4 months ago, created a question with more than 10,000 views. For Converting Protein Names To Gene Ids
Popular Question 4 months ago, created a question with more than 1,000 views. For RPKM to FPKM conversion
Great Question 6 months ago, created a question with more than 5,000 views. For GRCH38 and hg19
Popular Question 6 months ago, created a question with more than 1,000 views. For RPKM to FPKM conversion
Popular Question 8 months ago, created a question with more than 1,000 views. For RPKM to FPKM conversion
Great Question 8 months ago, created a question with more than 5,000 views. For GRCH38 and hg19
Epic Question 9 months ago, created a question with more than 10,000 views. For Converting Protein Names To Gene Ids
Popular Question 11 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 11 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 12 months ago, created a question with more than 1,000 views. For TopHat and Fastq with different Lane numbers
Popular Question 12 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 13 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 13 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 14 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 14 months ago, created a question with more than 1,000 views. For RPKM to FPKM conversion
Popular Question 14 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 15 months ago, created a question with more than 1,000 views. For Is Genotype Info Must While Validating Mutations
Popular Question 16 months ago, created a question with more than 1,000 views. For Is Genotype Info Must While Validating Mutations
Popular Question 16 months ago, created a question with more than 1,000 views. For Is Genotype Info Must While Validating Mutations
Popular Question 18 months ago, created a question with more than 1,000 views. For log ratio in gene expression array
Popular Question 18 months ago, created a question with more than 1,000 views. For RPKM to FPKM conversion
Popular Question 18 months ago, created a question with more than 1,000 views. For ncRNA gencode files
Popular Question 18 months ago, created a question with more than 1,000 views. For Is Genotype Info Must While Validating Mutations
Great Question 18 months ago, created a question with more than 5,000 views. For GRCH38 and hg19

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