User: timothy.kirkwood

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t***************@postgrad.manchester.ac.uk

Posts by timothy.kirkwood

<prev • 7 results • page 1 of 1 • next >
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Comment: C: IDT vs primer 3 - PCR dimerisation
... Cheers and sorry, I'll remember for the future! ...
written 6 days ago by timothy.kirkwood10
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Comment: C: MultiGeneBlast gui vs command prompt
... Cheers for the info! ...
written 6 days ago by timothy.kirkwood10
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Comment: A: MultiGeneBlast gui vs command prompt
... (reference) Marnix H. Medema, Eriko Takano, Rainer Breitling, Detecting Sequence Homology at the Gene Cluster Level with MultiGeneBlast, Molecular Biology and Evolution, Volume 30, Issue 5, May 2013, Pages 1218–1223, https://doi.org/10.1093/molbev/mst025 ...
written 6 days ago by timothy.kirkwood10
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MultiGeneBlast gui vs command prompt
... Hi Everyone! MultiGeneBlast searches for homologous collections of genes between an input genbank file and a .pal database of multiple genbank files, made using the MultiGeneBlast makedb function. I am trying to run MultiGeneBlast v 1.1.13 (win32) from https://sourceforge.net/projects/multigenebla ...
antismash v5 multigeneblast written 6 days ago by timothy.kirkwood10
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Answer: A: IDT vs primer 3 - PCR dimerisation
... I got a reply from IDT, in case anyone else has a similar issue - essentially they find all secondary structures and the structure TM to calculate the dG: Dear Dr Tirkwood, The tool does not use as input temperature but the parameter settings for the PCR or qPCR reaction are: Target type Oligo C ...
written 9 weeks ago by timothy.kirkwood10
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Comment: C: IDT vs primer 3 - PCR dimerisation
... Thanks for the advice, I have emailed them so can see what they say. Yes you're right, the problem is I'm doing an 8-plex PCR (4 primer pairs amplifying different gDNA regions) so I'm worried the interactions might be a bit more likely. Will see how it goes, wish me luck! ...
written 9 weeks ago by timothy.kirkwood10
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IDT vs primer 3 - PCR dimerisation
... I am in the process of screening a set of primers for hetero/homodimer formation. I have filtered 1200 primers using Primer3 (via it's python API - https://libnano.github.io/primer3-py/quickstart.html#thermodynamic-analysis), and now should have a set of primers that do not form homo/heterodimers ...
idt primer3 primer pcr written 10 weeks ago by timothy.kirkwood10

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Scholar 6 days ago, created an answer that has been accepted. For A: IDT vs primer 3 - PCR dimerisation

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