User: David_emir

gravatar for David_emir
David_emir380
Reputation:
380
Status:
Trusted
Location:
India
Last seen:
1 month ago
Joined:
7 years, 6 months ago
Email:
a************@gmail.com

about me

Posts by David_emir

<prev • 186 results • page 1 of 19 • next >
0
votes
1
answer
176
views
1
answers
Comment: C: Gene expression file to Expression matrix
... Thanks a lot, It worked like a charm ...
written 10 weeks ago by David_emir380
0
votes
1
answer
176
views
1
answers
Comment: C: Gene expression file to Expression matrix
... Thanks you RamRS will keep that in mind. ...
written 10 weeks ago by David_emir380
0
votes
1
answer
176
views
1
answers
Comment: C: Gene expression file to Expression matrix
... Thanks a lot, RamRS, it's such a great help. Going forward, I have tried your solution and used the following code bulk_clean<- tidyr::pivot_wider(bulk,id_cols=patient_id,gene_code) but i m not getting the desired output, please let me know where i am going wrong. thanks again. ...
written 10 weeks ago by David_emir380
2
votes
1
answer
176
views
1
answer
Gene expression file to Expression matrix
... Hi All, I have an RNAseq gene expression file (Count data) as follows, I need to conduct a Differential gene expression analysis between Patients, for that, I need to have this file as a Matrix of Rows as Genes and columns as counts (samples), how best can I get this as matrix file? Thanks a lot ...
rna-seq written 10 weeks ago by David_emir380 • updated 10 weeks ago by Alex Nesmelov170
0
votes
0
answers
161
views
0
answers
Comment: C: Quantification of cell cluster in Single cell data
... Thanks a lot for sharing such valuable information. Going forward, is there a way I can use my Seurat Object with CyTOF? ...
written 3 months ago by David_emir380
2
votes
0
answers
161
views
0
answers
Quantification of cell cluster in Single cell data
... Hi All, I have a single cell data from cancer patients and I have analysed the data using Seurat. Now, I wanted to do quantification of cell cluster frequency representation among patient groups. Many papers have used different methods. in this [paper][1], they have quantified the immune cell dist ...
wilcoxon rna-seq single cell seurat written 3 months ago by David_emir380
0
votes
0
answers
181
views
0
answers
Differentials Expressed Genes from each state in Monocle 2
... Hi there, I know that we can use differentialGeneTest to find genes that are most differentially expressed across the states by using the formula "~State". However, in the resulting data frame, there is no indication of the state number w.r.t. a given gene. Is it possible to get an extra column wi ...
dge monocle2 state single cell written 3 months ago by David_emir380
1
vote
1
answer
235
views
1
answer
CMS subtype identification in Single cell data
... Hi All, Is it possible to identify CMS subtypes for Single-cell data (Seurat object)? I am having ~ 50000 single cells data and using Seurat for the analysis. I am interested in CMS subtyping, is it possible to use CMScaller on single-cell and if yes how can I used Seurat object (counts) as an inpu ...
cmscaller single cell rna-seq written 4 months ago by David_emir380 • updated 4 months ago by jared.andrews078.3k
0
votes
1
answer
275
views
1
answers
Comment: C: Statistical test on Single-cell clusters(cell types)
... Thanks a lot for your help Kevin, so in. my case the inputs would be no of cells in a particular cell type , say CD4-TREG cell count in Normal would be my a=423 ? ...
written 5 months ago by David_emir380
0
votes
1
answer
275
views
1
answers
Comment: C: Statistical test on Single-cell clusters(cell types)
... Hi Kevin, Sorry for that not so clear question. I have 5-cell types identified (the excell snapshot is just for the illustration) - B-cell, Myeloid cell, T-cell, Epithelial, Stromal cell. and [according to this paper][1] they conducted Statistical comparisons of Gini coefficients across compartment ...
written 5 months ago by David_emir380

Latest awards to David_emir

Popular Question 6 weeks ago, created a question with more than 1,000 views. For Pre-processing And Quality Control of the Raw NGS Data -- > trimming
Popular Question 6 weeks ago, created a question with more than 1,000 views. For Pre-processing And Quality Control of the Raw NGS Data -- > trimming
Popular Question 8 weeks ago, created a question with more than 1,000 views. For Pre-processing And Quality Control of the Raw NGS Data -- > trimming
Popular Question 10 weeks ago, created a question with more than 1,000 views. For Pre-processing And Quality Control of the Raw NGS Data -- > trimming
Popular Question 11 weeks ago, created a question with more than 1,000 views. For rna seq public databases
Popular Question 12 weeks ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 4 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 5 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 5 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 6 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 8 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 8 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Voter 10 months ago, voted more than 100 times.
Popular Question 11 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 17 months ago, created a question with more than 1,000 views. For Quality control & Processing of RNA seq data
Popular Question 17 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis
Popular Question 17 months ago, created a question with more than 1,000 views. For Genomic Matrix data from TCGA need to be analysied for Differential gene expression
Popular Question 17 months ago, created a question with more than 1,000 views. For Pre-processing And Quality Control of the Raw NGS Data -- > trimming
Popular Question 17 months ago, created a question with more than 1,000 views. For What Types Of Analyses Are Typically Done After Snp Genotyping?
Popular Question 17 months ago, created a question with more than 1,000 views. For Error RNA-seq data, TOPHAT
Popular Question 17 months ago, created a question with more than 1,000 views. For rna seq public databases
Popular Question 18 months ago, created a question with more than 1,000 views. For Breast cancer TCGA data - DGE analysis

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1040 users visited in the last hour