User: MH85

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MH850
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Posts by MH85

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OrthoFiller - running time
... Hi everyone, does anyone have experience with the running time of OrthoFiller? I have one target genome ( ~ 700Mb) I would like to search, and 5 reference genomes - 24,524 orthogroups to process in total. Thanks a lot in advance Milos ...
genome annotation orthofiller orthologues written 9 days ago by MH850
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Comment: C: Maker Annotation - multiple gene models give same blastp match
... Thank you!!! I had no clue about this issue with -max_target_seqs 1 before. ...
written 12 days ago by MH850
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Maker Annotation - multiple gene models give same blastp match
... Hi everyone, INTRO: I have completed genome annotation of nonmodel organisms (fish) using MAKER. After three runs of MAKER, I filtered protein.fasta removing all with AED > 0.5 and I BLASTed these against SwissPort: `blastp -evalue 1e-6 -max_hsps 1 -max_target_seqs 1`. PROBLEM: I got quite a lo ...
genome annotation maker written 16 days ago by MH850 • updated 16 days ago by lieven.sterck10k

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