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User: Adrian Pelin

gravatar for Adrian Pelin
Adrian Pelin • 2.2k
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Canada
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Joined:
4 years, 11 months ago
Email:
a*******@uottawa.ca

Comparative and Population Genomics of Microsporidia. Comparative and Functional Genomics of Cryptomycotans.

Posts by Adrian Pelin

<prev • 473 results • page 1 of 48 • next >
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Comment: C: RNA-seq analysis of virally infected cells
... I do not mean to advertise products here, but when you say spike-in normalization, do you mean something like this: https://www.thermofisher.com/order/catalog/product/4456739 ? Are there better, maybe cheaper options out there? ...
written 28 days ago by Adrian Pelin • 2.2k
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Comment: C: RNA-seq analysis of virally infected cells
... What do you mean by global change? You mean that overall there is more viral genes in one condition compared to another, for instance more viral reads? ...
written 28 days ago by Adrian Pelin • 2.2k
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Comment: C: RNA-seq analysis of virally infected cells
... Duplicates, and multiple time points, but 12h is a more crucial one for us. ...
written 28 days ago by Adrian Pelin • 2.2k
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Answer: A: FPKM matrix after stringtie using ballgown
... Not sure you need ballgown to get FPKMs. My understanding is ballgown is needed for differential analysis, stringtie can output TPM and FPKM. When I run stringite, I pass the argument "-A output_dir/gene_abundances.tsv", and the gene_abundances.tsv has FPKM and TPM. Hope this helps, A ...
written 28 days ago by Adrian Pelin • 2.2k
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RNA-seq analysis of virally infected cells
... I have a human cancer cell line infected with 2 different vaccinia pox viruses (~200 kb dsDNA genome, 200 genes). At 12 hours post infection I harvested my RNA and send for RNA-seq. It looks like for strain_A, 40% reads are viral (60% host) while strain_B 60% reads are viral (40% host). This makes ...
host rna-seq virus written 28 days ago by Adrian Pelin • 2.2k • updated 28 days ago by Carlo Yague • 4.3k
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Comment: C: Anybody Managed To Make Inparanoid Work?
... I find orthoMCL to do the job better, that's why I gave up on inparanoid. ...
written 10 months ago by Adrian Pelin • 2.2k
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Comment: C: Detecting junctions from reads spanning large indels
... oh I see. Both still do the same thing though, correct? ...
written 11 months ago by Adrian Pelin • 2.2k
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Comment: C: Detecting junctions from reads spanning large indels
... sweet, goes directly to coordinates. Is there any way to get an intermediary sam file to see which reads were counted for generating coordinates? ...
written 11 months ago by Adrian Pelin • 2.2k
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Detecting junctions from reads spanning large indels
... Hello, I am mapping my reads with an aligner that allows for large indels (bbmap, other suggestions welcome). I am than using SamJs to get reads that have large indels >1kb java -jar ${HOME}/programs/jvarkit/dist/samjs.jar --file get_indel_sam_filter.javascript --samoutputformat SAM -o Fas ...
dna-seq sam written 11 months ago by Adrian Pelin • 2.2k • updated 11 months ago by Pierre Lindenbaum ♦ 112k
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Comment: C: Filter sam file to remove reads with large deletions
... Neat tool Pierre, I want to do the opposite, I want to find reads in sam file that span large deletions (>1kb), so I thought I would use your -X option, to save discarded. Running into a few issues, samoutputformat is not considered valid and -X doesn't get recognized? https://pastebin.com/k2dVRx ...
written 11 months ago by Adrian Pelin • 2.2k

Latest awards to Adrian Pelin

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