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1
vote
0
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320
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Comment:
Comment: Removing adapters in all the RNAseq samples regardless if they have or not, is i
10 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
778
views
Comment:
Comment: Installing Cell Ranger
10 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
485
views
Comment:
Comment: DESeq2 and normalization
11 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
677
views
Comment:
Comment: STAR aligner options
11 weeks ago by
dsull
★ 6.2k
1
vote
0
replies
1.0k
views
Comment:
Comment: Only one read per run - Trying to use CellRangerv7
11 weeks ago by
dsull
★ 6.2k
0
votes
1
reply
677
views
Comment:
Comment: STAR aligner options
11 weeks ago by
dsull
★ 6.2k
1
vote
1
reply
485
views
Answer:
Answer: DESeq2 and normalization
11 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
7.7k
views
Comment:
Comment: Differences between SMART-seq2, SMART-seq3, and 10x
11 weeks ago by
dsull
★ 6.2k
0
votes
1
reply
7.7k
views
Comment:
Comment: Differences between SMART-seq2, SMART-seq3, and 10x
11 weeks ago by
dsull
★ 6.2k
1
vote
1
reply
862
views
Comment:
Comment: cutadapt not trimming all adapters in PE Illumina sequencing
12 weeks ago by
dsull
★ 6.2k
1
vote
1
reply
862
views
Answer:
Answer: cutadapt not trimming all adapters in PE Illumina sequencing
12 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
282
views
Comment:
Comment: DEG (Differential expression gene) analysis
12 weeks ago by
dsull
★ 6.2k
0
votes
1
reply
282
views
Answer:
Answer: DEG (Differential expression gene) analysis
12 weeks ago by
dsull
★ 6.2k
3
votes
1
reply
437
views
Comment:
Comment: Average expression of a sample in single-cell data
12 weeks ago by
dsull
★ 6.2k
1
vote
0
replies
469
views
Answer:
Answer: scRNAseq normalization
12 weeks ago by
dsull
★ 6.2k
0
votes
0
replies
197
views
Answer:
Answer: STAR --sjdbGTFfile in alignment step
3 months ago by
dsull
★ 6.2k
1
vote
0
replies
390
views
Comment:
Comment: RNA-seq bam file normalization
3 months ago by
dsull
★ 6.2k
1
vote
1
reply
375
views
Answer:
Answer: scRNAseq output generated using STARsolo has gene_id rather than gene_name
3 months ago by
dsull
★ 6.2k
2
votes
0
replies
428
views
Comment:
Comment: How I should set `STAR` parameters to keep all the intronic reads?
3 months ago by
dsull
★ 6.2k
4
votes
1
reply
428
views
Answer:
Answer: How I should set `STAR` parameters to keep all the intronic reads?
3 months ago by
dsull
★ 6.2k
1
vote
1
reply
703
views
Comment:
Comment: Huge decrease of BUS records using bustools
3 months ago by
dsull
★ 6.2k
0
votes
1
reply
312
views
Comment:
Comment: Why does Phred+ encoding changes after trimming fastq files??
3 months ago by
dsull
★ 6.2k
3
votes
0
replies
666
views
Answer:
Answer: Would reaching out to local labs and startups help with my career transition?
3 months ago by
dsull
★ 6.2k
0
votes
0
replies
683
views
Answer:
Answer: Making PCA plot using variance instead of counts on Sleuth (plot_pca)
updated 3 months ago by
Ram
44k • written 3 months ago by
dsull
★ 6.2k
0
votes
0
replies
683
views
Comment:
Comment: Making PCA plot using variance instead of counts on Sleuth (plot_pca)
3 months ago by
dsull
★ 6.2k
2
votes
0
replies
602
views
Comment:
Comment: How does kallisto handle multi mapped reads?
3 months ago by
dsull
★ 6.2k
0
votes
0
replies
293
views
Answer:
Answer: Using tophat2 output for kallisto
3 months ago by
dsull
★ 6.2k
0
votes
2
replies
602
views
Comment:
Comment: How does kallisto handle multi mapped reads?
3 months ago by
dsull
★ 6.2k
2
votes
1
reply
436
views
Answer:
Answer: bulk seq vs. sc-seq
4 months ago by
dsull
★ 6.2k
0
votes
1
reply
657
views
Comment:
Comment: Differential expression analysis with very high duplication rate
4 months ago by
dsull
★ 6.2k
0
votes
1
reply
657
views
Answer:
Answer: Differential expression analysis with very high duplication rate
4 months ago by
dsull
★ 6.2k
1
vote
0
replies
464
views
Answer:
Answer: How to do DESeq2 on Kallisto Data
4 months ago by
dsull
★ 6.2k
0
votes
1
reply
537
views
Comment:
Comment: How to exclude sex-dependent genes from RNASEQ?
4 months ago by
dsull
★ 6.2k
1
vote
0
replies
537
views
Comment:
Comment: How to exclude sex-dependent genes from RNASEQ?
4 months ago by
dsull
★ 6.2k
1
vote
1
reply
749
views
Comment:
Comment: Why employ normalization methods, and how can they be utilized in DEG analysis?
4 months ago by
dsull
★ 6.2k
2
votes
1
reply
756
views
Comment:
Comment: RNA seq, correlation, gene expression, STAR count
4 months ago by
dsull
★ 6.2k
3
votes
1
reply
756
views
Answer:
Answer: RNA seq, correlation, gene expression, STAR count
4 months ago by
dsull
★ 6.2k
0
votes
0
replies
1.2k
views
Comment:
Comment: I would like to exclude rRNA in RSEM and quantify other genes.
4 months ago by
dsull
★ 6.2k
2
votes
1
reply
1.2k
views
Comment:
Comment: I would like to exclude rRNA in RSEM and quantify other genes.
4 months ago by
dsull
★ 6.2k
0
votes
0
replies
146k
views
Comment:
Comment: The Biostar Handbook. A bioinformatics e-book for beginners.
4 months ago by
dsull
★ 6.2k
1
vote
1
reply
1.2k
views
Answer:
Answer: I would like to exclude rRNA in RSEM and quantify it.
4 months ago by
dsull
★ 6.2k
0
votes
0
replies
1.2k
views
Comment:
Comment: Troubleshooting RNA-seq data with DNA contamination
4 months ago by
dsull
★ 6.2k
1
vote
1
reply
1.2k
views
Comment:
Comment: Troubleshooting RNA-seq data with DNA contamination
4 months ago by
dsull
★ 6.2k
0
votes
1
reply
1.2k
views
Comment:
Comment: Troubleshooting RNA-seq data with DNA contamination
4 months ago by
dsull
★ 6.2k
0
votes
1
reply
1.2k
views
Comment:
Comment: Troubleshooting RNA-seq data with DNA contamination
4 months ago by
dsull
★ 6.2k
2
votes
2
replies
1.2k
views
Comment:
Comment: Can I estimate RNA velocity in Visium Spatial Gene Expression for FFPE
5 months ago by
dsull
★ 6.2k
1
vote
1
reply
1.2k
views
Comment:
Comment: Can I estimate RNA velocity in Visium Spatial Gene Expression for FFPE
5 months ago by
dsull
★ 6.2k
0
votes
0
replies
2.4k
views
Comment:
Comment: Determining computer time required
5 months ago by
dsull
★ 6.2k
0
votes
0
replies
723
views
Comment:
Comment: Problem in Salmon when indexing Rattus_norvegicus
5 months ago by
dsull
★ 6.2k
1
vote
0
replies
2.4k
views
Comment:
Comment: Determining computer time required
5 months ago by
dsull
★ 6.2k
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