How miRNA sequencing works by Illumina GA ?
0
3
Entering edit mode
10.5 years ago
jack ▴ 980

Hi all,

I'm using TCGA data set to analysis the expression of miRNA in cancers. They used Illumina HiSeq to sequence the micorRNAs. now the question for me is that, they sequenced all miRNAs (miRNA precursores, mature miRNA, miRNA stem loop ), or they just isolated mature miRAN from Gel electrophoresis and sequenced it ? because I cheecked some of the IDs of miRNAs at TCGA data set via miRBase database, some of them seems to belong to miRNA precursore or miRNA stem loop.

Also I looked at the META data file of miRNA at TCGA, they describe like this:

Ligation of linkers and reverse transcription of small RNAs PCR with sequencing primers, size fractionation Sequencing on Illumina GAIIx Alignment of reads to reference genome Read counts per mirna isoform Normalized expression per mirna gene SDRF Files

RNA-Seq sequence sequencing next-gen • 3.2k views
ADD COMMENT

Login before adding your answer.

Traffic: 1582 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6