Amount of mappable circulating DNA
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8.7 years ago
Phil S. ▴ 700

Hi guys,

Does any one of you have an Idea / a link to a reference how much of circulating DNA should be mappable to the human reference genome (at the moment I don't care which version of it).

Thanks, in advance!

Best,
Phil

mapping human DNA • 1.7k views
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If the dna source is human, it will map like any other. If the dna source is non-human, that will probably vary a lot on a case-by-case basis.

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so basically you mean that you are able to map like 95% of reads coming from human CNAPS to the human reference genome?

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8.5 years ago

Hello!

I believe the answer to your question has a strong dependence on sample quality and source (serum/plasma) and library preparation procedure.

The literature contains some info on your question. See e.g. this paper, especially Table 2 with around 10% unaligned and 20% ambiguously mapped sequences

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