Does anyone know why the error occurred when I just make a comparison of 1 time point (2hHGF) sample and 1 time point (2hnoHGF) sample? The error is shown in yellow below. And how to fix the error?
> contrast.matrix<-makeContrasts(2hHGF-2hnoHGF, levels=design)
Error in ebayes(fit = fit, proportion = proportion, stdev.coef.lim = stdev.coef.lim, :
No residual degrees of freedom in linear model fits