Question: phylogeny tree after a blastx run
0
gravatar for arronar
4.9 years ago by
arronar160
Austria
arronar160 wrote:

Hi!

I just run a blastx , using a nucleotide sequence and 192 Blast Hits returned. What i want to do is to create a phylogeny tree to see which organism from blast's results are closer genetically to my query.

1) Is there any way to create that tree through NCBI's site after blastx run ?

2) What i've tried is that i downloaded all the sequences (Hits), in fasta format, and ran a multiple sequence alignment using Jalview. The problem here is that in fasta file , my query sequence didn't exist so i couldn't conclude something.

Is the way i am approaching it right ? How can i find the amino-acid sequence of my query and add in the fasta file ? Do you suggest me another way to achieve that goal ?

 

Thanks.

blast tree alignment phylogeny • 1.6k views
ADD COMMENTlink modified 4.0 years ago by ubai0 • written 4.9 years ago by arronar160
0
gravatar for ubai
4.0 years ago by
ubai0
ubai0 wrote:

Hi

If you are still having this issue, try this "Building Phylogenetic Trees from Molecular Data with MEGA" its a paper describing a full straight forward protocol.

Good luck

ADD COMMENTlink written 4.0 years ago by ubai0
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