I just run a blastx , using a nucleotide sequence and 192 Blast Hits returned. What i want to do is to create a phylogeny tree to see which organism from blast's results are closer genetically to my query.
1) Is there any way to create that tree through NCBI's site after blastx run ?
2) What i've tried is that i downloaded all the sequences (Hits), in fasta format, and ran a multiple sequence alignment using Jalview. The problem here is that in fasta file , my query sequence didn't exist so i couldn't conclude something.
Is the way i am approaching it right ? How can i find the amino-acid sequence of my query and add in the fasta file ? Do you suggest me another way to achieve that goal ?