phylogeny tree after a blastx run

Question

[arronar]

Hi!

I just run a blastx using a nucleotide sequence and 192 Blast Hits returned. What I want to do is to create a phylogeny tree to see which organism from blast's results are closer genetically to my query.

1. Is there any way to create that tree through NCBI's site after blastx run?
2. What I've tried is that I downloaded all the sequences (Hits), in fasta format, and ran a multiple sequence alignment using Jalview. The problem here is that in fasta file, my query sequence didn't exist so I couldn't conclude something.

Is the way I am approaching it right? How can I find the amino-acid sequence of my query and add in the fasta file? Do you suggest me another way to achieve that goal?

Thanks

Answer 1

[ubai]

Hi

If you are still having this issue, try this "Building Phylogenetic Trees from Molecular Data with MEGA" its a paper describing a full straight forward protocol.

Good luck