Question: Pipeline for analysing multiple tumor/normal exome samples
1
gravatar for Nick
4.8 years ago by
Nick260
Spain
Nick260 wrote:

I have a set of biological replicates consisting of multiple normal and tumor mouse samples. The samples are not matched (each sample corresponds to a different animal) but these are all mice from the same litter and are supposed to be genetically identical (apart from the somatic mutations in tumors). 

I am interested in a pipeline that would produce joint analysis of all samples. All the pipelines/tutorials I've seen are about single tumor/normal pairs. In theory, these can be re-used for multiple replicates by looking at overlapping mutations. But this seems to me to be a bit primitive. Also, in my case the samples are not matched so maybe it makes sense to consider some sort of cohort/pooled analysis. 

I am facing such an experimental design for the first time so would welcome any suggestions. 

tumor exome • 1.7k views
ADD COMMENTlink modified 4.8 years ago by donfreed1.4k • written 4.8 years ago by Nick260
1
gravatar for donfreed
4.8 years ago by
donfreed1.4k
Mountain View, CA
donfreed1.4k wrote:

Try deepSNV. It is not built exactly for your situation but it might be more applicable than MuTect or Strelka.

ADD COMMENTlink written 4.8 years ago by donfreed1.4k
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