Purifying selection M7 and M8 models

Question

[RB]

Dear All,

Can anyone suggest me how to utilize m7 and m8 models results to estimated purifying selection or relaxed constraints acting on your gene family.

Any other tutorial or methodology will also be helpful.

Thanks,
Reb

Answer 1

[Martombo]

You can use the program codeml from the package PAML, manual here

If you're new to programming you might find it easier to use the biopython implementation of PAML, it provides quite a user friendly interface to it and there are also examples you can follow.