Can anyone tell me the difference between the frequency output provided by the VarScan? I know one is for normal and other is for the variant allele in tumor. But is this frequency for the Variant allele likely said in website of the tool the global VAF or is it the VAF for the individual in the matched normal to control. Am a bit confused with that. If I have to consider for the somatic and germline events and its corresponding frequency of variant allele I should consider this frequency but can I term it as global VAF or is it population VAF? If not global VAF how should I calculate the global VAF? If I considered a published literature datasets with 400 datasets where the same variants is mentioned with a VAF value will that be global VAF and can be compared to my dataset whose VAF should be for the individual population? I am a bit confused but since am not getting some important cancer genes from my samples (which is 1 N/T for low grade cancer and 1 N/T for high grade cancer) I have to device a strategy to list out the driver and passenger mutations from this using both germlline and somatic variations. I would appreciate any suggestions given by the community.