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• analysis amplicon miseq
  Hi all，I sequenced dozens of genes in tumor-normal samples using truseq custom amplicon kit on Mi...
• VarDict Amplicon-Aware Calling with barcoded seq data
  I'm trying out different variant calling software on non-matched somatic samples, where the pcr h...
• Removing PCR primers in targeted sequencing with nested amplicons
  hi everyone, I am analyzing a 150x2 MiSeq data where there are multiple amplicons from a gene bu...
• Depth of coverage for specific target from bam file
  Hi all, We used multiple PCR to prepare the amplicons and loaded on MiSeq to get the sequencing ...
• can anyone give some example codes to remove primer sequence from MiSeq PCR amplicon sequences
  Hi, I have some illumina MiSeq paired end sequence data to process. the data are from PCR sample...
• processing amplicon seq data from miseq
  I have .fastq files from a targeted amplicon seq run on MiSeq using Illumina library prep workflo...
• Training dataset for NGS HLA typing (reads >200bp from PCR amplicons)
  I'm looking for a training set from human HLA typing with long reads (454, IonTorrent or MiSeq 30...
• Read Count Differences in Amplicon Sequencing
  I have a question about differences in read count for Illumina MISeq data. I am attempting to seq...
• Best pratices for variant calling on deep sequencing TruSeq Custom Amplicon data
  Hi， I'm trying to analyze a panel of genes in tumor samples using truseq custom amplicon (TSCA) ...
• Analysis of NGS data from a Long-PCR product
  Hello everyone! I am faced with something I haven't tried before, and I am unsure how to proceed...
• allelic unbalance for multiplex PCR based amplicon data
  Hi all, We used multiplex PCR to enrich the target regions, and then get them sequenced on HiSeq...
• Error rate in MiSeq targeted amplicons and calculating the allele frequency
  I am aligning MiSeq deep sequencing targeted amplicons to human genome (paired ended). The resul...
• Identifying FLT3-ITD with Pindel
  Hi everyone, Looking to see if anyone else has any experience in looking for somatic mutations i...
• amplicon 16S reads qualityc check
  Hi, community, I have done my amplicon 16S illumina miseq sequencing. My samples were from waste...
• The first amplicon librariesis is needed to analyse the 16S data?
  Hi, For analyzing 16S data with Mothur or Qiime is it necessary that I have the the first amplic...
• GemSim Grinder and MetaSim to simulate 16s rRNA libraries
  Hello everyone, What we are trying to achieve is to detect the existence of interested microbial...
• Pipeline For Amplicons Analysis
  Hi all! Could you recommend any pipelines for analysis of amplicons ? Is supposed that we will a...
• Underestimating heterozygotes in variant calling in FreeBayes
  Hello, I have discovered a problem with underestimating heterozygous sites in my samples. I hav...
• Multiplex PCR Primer Design tool
  [PanelPlex™][1] Provides completely automated design of multiplex PCR with unprecedented covera...
• calling phased variants
  Hi All I’m trying to call phased variants in 400-450bp amplicons derived from pooled samples tha...
• CpG Methylation data from PacBio sequencing
  Sorry if this is a dumb question, but I am told by our sequencing core that you cannot detect met...