Question: Power estimate for multiple timepoint expression study
1
gravatar for coleman_jonathan
5.4 years ago by
European Union
coleman_jonathan420 wrote:

I am planning a study examining genome-wide gene expression changes across three timepoints (baseline, following a treatment, and some time after a treatment).

Does anyone know of any statistical package or equation to estimate the effect sizes a longitudinal analysis of this would be powered to discover, given a known sample size and modelling the data as a mixed linear model incorporating the effect of time?

ADD COMMENTlink modified 5.4 years ago by Devon Ryan92k • written 5.4 years ago by coleman_jonathan420
1
gravatar for Devon Ryan
5.4 years ago by
Devon Ryan92k
Freiburg, Germany
Devon Ryan92k wrote:

Normal power calculations would be for just one of the genes, so you end up needing to use another dataset as a model for how yours will behave and then determining power according to that. One popular tool for doing that with RNAseq is Scotty. These generally work better if you've already done a pilot experiment (as is true with all power analyses, since otherwise you're really just making numbers up).

ADD COMMENTlink written 5.4 years ago by Devon Ryan92k
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