I have exome data of 25 unrelated patients and 40 unrelated control samples. I'm looking for rare variants associated with the disease.
I've checked some obvious things: one variant present in cases not in controls. One gene enriched for rare variants in cases compared to controls.
However, now I want to do some statistical testing to find association of a variant/gene with the disease. Since this is just a small-scale study I don't think I can work with methods used in GWAS. Can someone point me to some papers/methods/ideas that could be of interest for my specific situation?
Thanks in advance