Any one used DIAG grid resource to run trinity? I am wondering what is maximum RAM request in grid.
Since trinity recommends to combine all your samples from different conditions and construct only one transcriptome for expression analysis, I want to combine my 36 samples (each sample can be 50M to 100M) reads. It seems I need 4TB RAM if I followed 1GB per 1M reads standard.
Also, I am wondering whether normalization will compromise the assembly quality a lot or not, especially considering that I want to detect low expressed factors.