Question: RAM limitation on trinity using DIAG computational resource
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gravatar for sckinta
5.3 years ago by
sckinta550
United States
sckinta550 wrote:

Hi, 

Any one used DIAG grid resource to run trinity? I am wondering what is maximum RAM request in grid. 

Since trinity recommends to combine all your samples from different conditions and construct only one transcriptome for expression analysis, I want to combine my 36 samples (each sample can be 50M to 100M) reads. It seems I need 4TB RAM if I followed 1GB per 1M reads standard. 

Also, I am wondering whether normalization will compromise the assembly quality a lot or not, especially considering that I want to detect low expressed factors. 

Thank you

rna-seq ram diag • 1.1k views
ADD COMMENTlink written 5.3 years ago by sckinta550
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