Entering edit mode
9.7 years ago
niu2rseq
▴
90
Hello,
I have some microRNAseq data and I want to compare those with the miRbase. I am using miRExpress. Since for the animal I used here they don't have a specific miRNA database, I just downloaded all the data from all species and merge them togehter. I call it all_precursor.txt.
This database mach failed (in miRExpress it's called alignmentSIMD), following is the error information.
I guess the problem is it contains some ambiguous nucleotide like B here. Dear fellows, how can I fix this problem? I am new to this RNAseq area. Thank you very much!
Illegal character! UGGUAUGGGCAUUGUUGCCAAUACAGGAGAACGUGGBUCUUUUUGACAUAAUGCCUAGAACUACCCAUAGCUCCAUCCCAACUCAUAAAUAGGAGGAUAAGACUGAAUUCACGUCCUCCUAUACUGGUAACAAUGCGCAUGCCAA
