Hi Everyone,
I am a complete beginner here who has landed himself with some metatranscriptomic data from some mice gut samples. All the sequencing was already done, but I am now left with raw sequences and a bit confused at how to proceed. I know the general gist of what I want to do, but am having a problem with the actual implementation. I am planning on using the methods from this paper.
In short I am having a problem setting up and annotating a reference database. I want to take my sequences from the HMP but I don't know which fasta files I need (I am guessing ASM format). Then when I need to annotate those genomes I am supposed to use BlastX against the KEGG database, but do I need to pay for it or is there another way? Any help would be appreciated.
