how to extract rna seq data for bacteria.. urgent help needed...

Question

[prachi1627]

hi,

i want to extract rna seq data for bacteria group. please tell me how i can get it.. i need it urgently...

Answer 1

[Devon Ryan]

Really? You didn't even try did you? Note the links to a bunch of GEO datasets in the supplemental data (hint, you could have just search GEO yourself).

[prachi1627]

i tried but i m not able to understand it, they are like related to specific condition. for my work i need complete genome expression data. i am very new to this rna seq, i don't know how to use this cufflink and r bioconductor ... i am looking for terminator region...in each gene expression so i need complet transcript from rna seq data ..

[Devon Ryan]

Everything is going to be related to a specific condition, even "normal" is a condition, though it may not correspond to the normal you have in mind. If you're looking for terminator regions then you probably want to think about the molecular biology of how to find one and then look and see if there's a dataset appropriate for that. It's quite likely that you'll need to just do the experiment to get what you want.

BTW, have a read through this paper if you need help with that.

[prachi1627]

i am from the lab, where Webgester is developed , working on same project

[Devon Ryan]

Then you should be able to just ask your colleagues about this.

[prachi1627]

see its very simple , if i am having information from my colleagues why i will post here... n rather than all this if u can answer my query i will wil be great full.. ..

[Devon Ryan]

You already have all the information you need; I will provide no further help.

[Prakki Rama]

Sorry. may be off-topic comment. But felt funny with the lmgtfy link and curious. How did lmgtfy link got redirected to directly to RNASeq paper instead of google search?

[prachi1627]

thanks for ur reply...

[Devon Ryan]

You can choose the "I'm feeling luck" button, just like with google.